SMG7 Protein (AA 2-1137) (His tag)
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- Antigène Voir toutes SMG7 Protéines
- SMG7 (Smg-7 Homolog, Nonsense Mediated mRNA Decay Factor (SMG7))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-1137
- Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette SMG7 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS), Crystallization (Crys), ELISA, Western Blotting (WB)
- Séquence
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SLQSAQYLRQ AEVLKADMTD SKLGPAEVWT SRQALQDLYQ KMLVTDLEYA LDKKVEQDLW NHAFKNQITT LQGQAKNRAN PNRSEVQANL SLFLEAASGF YTQLLQELCT VFNVDLPCRV KSSQLGIISN KQTHTSAIVK PQSSSCSYIC QHCLVHLGDI ARYRNQTSQA ESYYRHAAQL VPSNGQPYNQ LAILASSKGD HLTTIFYYCR SIAVKFPFPA ASTNLQKALS KALESRDEVK TKWGVSDFIK AFIKFHGHVY LSKSLEKLSP LREKLEEQFK RLLFQKAFNS QQLVHVTVIN LFQLHHLRDF SNETEQHTYS QDEQLCWTQL LALFMSFLGI LCKCPLQNES QEESYNAYPL PAVKVSMDWL RLRPRVFQEA VVDERQYIWP WLISLLNSFH PHEEDLSSIS ATPLPEEFEL QGFLALRPSF RNLDFSKGHQ GITGDKEGQQ RRIRQQRLIS IGKWIADNQP RLIQCENEVG KLLFITEIPE LILEDPSEAK ENLILQETSV IESLAADGSP GLKSVLSTSR NLSNNCDTGE KPVVTFKENI KTREVNRDQG RSFPPKEVRR DYSKGITVTK NDGKKDNNKR KTETKKCTLE KLQETGKQNV AVQVKSQTEL RKTPVSEARK TPVTQTPTQA SNSQFIPIHH PGAFPPLPSR PGFPPPTYVI PPPVAFSMGS GYTFPAGVSV PGTFLQPTAH SPAGNQVQAG KQSHIPYSQQ RPSGPGPMNQ GPQQSQPPSQ QPLTSLPAQP TAQSTSQLQV QALTQQQQSP TKAVPALGKS PPHHSGFQQY QQADASKQLW NPPQVQGPLG KIMPVKQPYY LQTQDPIKLF EPSLQPPVMQ QQPLEKKMKP FPMEPYNHNP SEVKVPEFYW DSSYSMADNR SVMAQQANID RRGKRSPGVF RPEQDPVPRM PFEKSLLEKP SELMSHSSSF LSLTGFSLNQ ERYPNNSMFN EVYGKNLTSS SKAELSPSMA PQETSLYSLF EGTPWSPSLP ASSDHSTPAS QSPHSSNPSS LPSSPPTHNH NSVPFSNFGP IGTPDNRDRR TADRWKTDKP AMGGFGIDYL SATSSSESSW HQASTPSGTW TGHGPSMEDS SAVLMESLKS IWSSSMMHPG PSALEQLLMQ QKQKQQRGQG TMNPPH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human SMG7 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product SMG7 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- SMG7 (Smg-7 Homolog, Nonsense Mediated mRNA Decay Factor (SMG7))
- Autre désignation
- SMG7 (SMG7 Produits)
- Synonymes
- 9430023P16Rik Protein, mKIAA0250 Protein, C1orf16 Protein, EST1C Protein, SGA56M Protein, Smg-7 homolog, nonsense mediated mRNA decay factor (C. elegans) Protein, SMG7, nonsense mediated mRNA decay factor Protein, Smg7 Protein, SMG7 Protein
- Sujet
- Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. {ECO:0000269|PubMed:15546618, ECO:0000269|PubMed:15721257}.
- Poids moléculaire
- 128.1 kDa Including tag.
- UniProt
- Q92540
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