SPIDR Protein (AA 1-915) (Strep Tag)
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- Antigène Tous les produits SPIDR
- SPIDR (Scaffolding Protein Involved in DNA Repair (SPIDR))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-915
- Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette SPIDR protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MPRGSRARGS KRKRSWNTEC PSFPGERPLQ VRRAGLRTAG AAASLSEAWL RCGEGFQNTS GNPSLTAEEK TITEKHLELC PRPKQETTTS KSTSGLTDIT WSSSGSDLSD EDKTLSQLQR DELQFIDWEI DSDRAEASDC DEFEDDEGAV EISDCASCAS NQSLTSDEKL SELPKPSSIE ILEYSSDSEK EDDLENVLLI DSESPHKYHV QFASDARQIM ERLIDPRTKS TETILHTPQK PTAKFPRTPE NSAKKKLLRG GLAERLNGLQ NRERSAISLW RHQCISYQKT LSGRKSGVLT VKILELHEEC AMQVAMCEQL LGSPATSSSQ SVAPRPGAGL KVLFTKETAG YLRGRPQDTV RIFPPWQKLI IPSGSCPVIL NTYFCEKVVA KEDSEKTCEV YCPDIPLPRR SISLAQMFVI KGLTNNSPEI QVVCSGVATT GTAWTHGHKE AKQRIPTSTP LRDSLLDVVE SQGAASWPGA GVRVVVQRVY SLPSRDSTRG QQGASSGHTD PAGTRACLLV QDACGMFGEV HLEFTMSKAR QLEGKSCSLV GMKVLQKVTR GRTAGIFSLI DTLWPPAIPL KTPGRDQPCE EIKTHLPPPA LCYILTAHPN LGQIDIIDED PIYKLYQPPV TRCLRDILQM NDLGTRCSFY ATVIYQKPQL KSLLLLEQRE IWLLVTDVTL QTKEERDPRL PKTLLVYVAP LCVLGSEVLE ALAGAAPHSL FFKDALRDQG RIVCAERTVL LLQKPLLSVV SGASSCELPG PVMLDSLDSA TPVNSICSVQ GTVVGVDEST AFSWPVCDMC GNGRLEQRPE DRGAFSCGDC SRVVTSPVLK RHLQVFLDCR SRPQCRVKVK LLQRSISSLL RFAAGEDGSY EVKSVLGKEV GLLNCFVQSV TAHPTSCIGL EEIELLSAGG ASAEH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- SPIDR (Scaffolding Protein Involved in DNA Repair (SPIDR))
- Autre désignation
- SPIDR (SPIDR Produits)
- Synonymes
- KIAA0146 Protein, 2310008H04Rik Protein, Kiaa0146 Protein, RGD1566036 Protein, scaffolding protein involved in DNA repair Protein, SPIDR Protein, Spidr Protein
- Sujet
- DNA repair-scaffolding protein (Scaffolding protein involved in DNA repair),FUNCTION: Plays a role in DNA double-strand break (DBS) repair via homologous recombination (HR). Serves as a scaffolding protein that helps to promote the recruitment of DNA-processing enzymes like the helicase BLM and recombinase RAD51 to site of DNA damage, and hence contributes to maintain genomic integrity. {ECO:0000269|PubMed:23509288, ECO:0000269|PubMed:23754376, ECO:0000269|PubMed:27967308, ECO:0000269|PubMed:34697795}.
- Poids moléculaire
- 100.3 kDa
- UniProt
- Q14159
- Pathways
- Positive Regulation of Response to DNA Damage Stimulus
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