USP5 Protein (AA 2-858) (His tag)
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- Antigène Voir toutes USP5 Protéines
- USP5 (Ubiquitin Specific Peptidase 5 (USP5))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-858
- Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette USP5 protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
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AELSEEALLS VLPTIRVPKA GDRVHKDECA FSFDTPESEG GLYICMNTFL GFGKQYVERH FNKTGQRVYL HLRRTRRPKE EDPATGTGDP PRKKPTRLAI GVEGGFDLSE EKFELDEDVK IVILPDYLEI ARDGLGGLPD IVRDRVTSAV EALLSADSAS RKQEVQAWDG EVRQVSKHAF SLKQLDNPAR IPPCGWKCSK CDMRENLWLN LTDGSILCGR RYFDGSGGNN HAVEHYRETG YPLAVKLGTI TPDGADVYSY DEDDMVLDPS LAEHLSHFGI DMLKMQKTDK TMTELEIDMN QRIGEWELIQ ESGVPLKPLF GPGYTGIRNL GNSCYLNSVV QVLFSIPDFQ RKYVDKLEKI FQNAPTDPTQ DFSTQVAKLG HGLLSGEYSK PVPESGDGER VPEQKEVQDG IAPRMFKALI GKGHPEFSTN RQQDAQEFFL HLINMVERNC RSSENPNEVF RFLVEEKIKC LATEKVKYTQ RVDYIMQLPV PMDAALNKEE LLEYEEKKRQ AEEEKMALPE LVRAQVPFSS CLEAYGAPEQ VDDFWSTALQ AKSVAVKTTR FASFPDYLVI QIKKFTFGLD WVPKKLDVSI EMPEELDISQ LRGTGLQPGE EELPDIAPPL VTPDEPKGSL GFYGNEDEDS FCSPHFSSPT SPMLDESVII QLVEMGFPMD ACRKAVYYTG NSGAEAAMNW VMSHMDDPDF ANPLILPGSS GPGSTSAAAD PPPEDCVTTI VSMGFSRDQA LKALRATNNS LERAVDWIFS HIDDLDAEAA MDISEGRSAA DSISESVPVG PKVRDGPGKY QLFAFISHMG TSTMCGHYVC HIKKEGRWVI YNDQKVCASE KPPKDLGYIY FYQRVAS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human USP5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product USP5 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- USP5 (Ubiquitin Specific Peptidase 5 (USP5))
- Autre désignation
- USP5 (USP5 Produits)
- Synonymes
- ISOT Protein, AA407472 Protein, ISOT-1 Protein, Ucht Protein, ubiquitin specific peptidase 5 Protein, ubiquitin carboxyl-terminal hydrolase 5 Protein, ubiquitin carboxyl-terminal hydrolase 5, putative Protein, ubiquitin specific protease 5 Protein, ubiquitin specific peptidase 5 (isopeptidase T) Protein, USP5 Protein, CNH02890 Protein, EHI_049940 Protein, CC1G_12961 Protein, MCYG_01862 Protein, CGB_L3600C Protein, PY00546 Protein, Usp5 Protein
- Sujet
- Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition. {ECO:0000269|PubMed:19098288}.
- Poids moléculaire
- 96.6 kDa Including tag.
- UniProt
- P45974
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