ZNF541 Protein (AA 1-1346) (Strep Tag)
-
- Antigène Tous les produits ZNF541
- ZNF541 (Zinc Finger Protein 541 (ZNF541))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1346
- Origine
- Humain
- Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ZNF541 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
-
MDQYSLGDEG ALPSEMHLPS FSESQGLNCS DTLNRDLGPN TRGFLYAGLS GLDPDPSLPT PDMSSEVLED NLDTLSLYSG KDSDSVKLLE EYADSESQAS LQDLGLGVLK AKEADEGGRA TSGSARKGKR QHSSPQNPLL DCSLCGKVFS SASSLSKHYL THSQERKHVC KICSKAFKRQ DHLTGHMLTH QKTKPFVCIE QGCSKSYCDY RSLRRHYEVH HGLCILKEAP PEEEACGDSP HAHESAGQPP PSSLRSLVPP EARSPGSLLP HRDLLRRIVS SIVHQKTPSP GPAPAGASDS EGRNTACPCP ASSGSSSCTP AGPHAAPAAL DTELPEEPCL PQKEPATDVF TAPNSRAAEN GAPDPPEPEP DTALLQARST AECWPEGGSV PACLPLFRGQ TVPASSQPSS HSFQWLRNLP GCPKSKGNNV FVVHKPSAVP SREGSESGPG PSSGSPSEES PPGPGGGLED ALPFPAALLR VPAEAPSDPR SASGEDDPCA PKKVKVDCDS FLCQNPGEPG LQEAQKAGGL PADASPLFRQ LFLKSQEPLV SHEQMQVFQM ITKSQRIFSH AQVAAVSSQL PAPEGKPAAL RPLQGPWPQQ PPPLAPAVDS LHAGPGNPEA EGSPARRRKT TPGVPREASP GSTRRDAKGG LKVAAVPTPL AAPSLDPSRN PDISSLAKQL RSSKGTLDLE DIFPSTGQRQ TQLGGEEPPG ASLPGKQAPA ENGAASRITK GEKGPACSRG GGYRLLGNPR APRFSGFRKE KAKMDMCCAA SPSQVAMASF SSAGPPADPS KSKLTIFSRI QGGNIYRLPH PVKEENVAGR GNQQNGSPTD WTKPRSTFVC KNCSQMFYTE KGLSSHMCFH SDQWPSPRGK QEPQVFGTEF CKPLRQVLRP EGDRHSPPGT KKPLDPTAAA PLVVPQSIPV VPVTRHIGSM AMGQEKDGEE RDSKESSQQR KRKKRPPPST AGEPGPAGCH QSRLRSPMFL VDCLLKGLFQ CSPYTPPPML SPIREGSGVY FNTLCSTSTQ ASPDQLISSM LDQVDGSFGI CVVKDDTKIS IEPHINIGSR FQAEIPELQE RSLAGTDEHV ASLVWKPWGD MMISSETQDR VTELCNVACS SVMPGGGTNL ELALHCLHEA QGNVQVALET LLLRGPHKPR THLLADYRYT GSDVWTPIEK RLFKKAFYAH KKDFYLIHKM IQTKTVAQCV EYYYIWKKMI KFDCGRAPGL EKRVKREPEE VERTEEKVPC SPRERPSHHP TPKLKTKSYR RESILSSSPN AGSKRTPELL GSAESQGIFP CRECERVFDK IKSRNAHMKR HRLQDHVEPI IRVKWPVKPF QLKEEELGAD IGPLQW
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- ZNF541 (Zinc Finger Protein 541 (ZNF541))
- Autre désignation
- ZNF541 (ZNF541 Produits)
- Synonymes
- ZNF541 Protein, EG666528 Protein, Znf541 Protein, zinc finger protein 541 Protein, ZNF541 Protein, Zfp541 Protein
- Sujet
- Zinc finger protein 541,FUNCTION: Transcription regulator which is essential for male fertility and for the completion of meiotic prophase in spermatocytes. Regulates progression of the pachytene stage of meiotic prophase by activating the expression of genes involved in meiosis during spermatogenesis. Maintains the repression of pre-pachytene transcriptional programs, including meiotic double-strand breaks (DSB) formation genes in pachytene spermatocytes and suppresses aberrant DSB formation after mid-pachytene, thus ensuring meiosis progression. {ECO:0000250|UniProtKB:Q0GGX2}.
- Poids moléculaire
- 145.6 kDa
- UniProt
- Q9H0D2
-