SLC28A2 Protein (AA 1-658) (Strep Tag)
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- Antigène Voir toutes SLC28A2 Protéines
- SLC28A2 (Solute Carrier Family 28 (Sodium-Coupled Nucleoside Transporter), Member 2 (SLC28A2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-658
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette SLC28A2 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MEKASGRQSI ALSTVETGTV NPGLELMEKE VEPEGSKRTD AQGHSLGDGL GPSTYQRRSR WPFSKARSFC KTHASLFKKI LLGLLCLAYA AYLLAACILN FQRALALFVI TCLVIFVLVH SFLKKLLGKK LTRCLKPFEN SRLRLWTKWV FAGVSLVGLI LWLALDTAQR PEQLIPFAGI CMFILILFAC SKHHSAVSWR TVFSGLGLQF VFGILVIRTD LGYTVFQWLG EQVQIFLNYT VAGSSFVFGD TLVKDVFAFQ ALPIIIFFGC VVSILYYLGL VQWVVQKVAW FLQITMGTTA TETLAVAGNI FVGMTEAPLL IRPYLGDMTL SEIHAVMTGG FATISGTVLG AFIAFGVDAS SLISASVMAA PCALASSKLA YPEVEESKFK SEEGVKLPRG KERNVLEAAS NGAVDAIGLA TNVAANLIAF LAVLAFINAA LSWLGELVDI QGLTFQVICS YLLRPMVFMM GVEWTDCPMV AEMVGIKFFI NEFVAYQQLS QYKNKRLSGM EEWIEGEKQW ISVRAEIITT FSLCGFANLS SIGITLGGLT SIVPHRKSDL SKVVVRALFT GACVSLISAC MAGILYVPRG AEADCVSFPN TSFTNRTYET YMCCRGLFQS TSLNGTNPPS FSGPWEDKEF SAMALTNCCG FYNNTVCA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product SLC28A2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- SLC28A2 (Solute Carrier Family 28 (Sodium-Coupled Nucleoside Transporter), Member 2 (SLC28A2))
- Autre désignation
- SLC28A2 (SLC28A2 Produits)
- Synonymes
- SLC28A1 Protein, cnt2 Protein, spnt1 Protein, MGC82237 Protein, CNT2 Protein, HCNT2 Protein, HsT17153 Protein, SPNT1 Protein, 2010208B10Rik Protein, B430217P18 Protein, BB152493 Protein, Cnt2 Protein, solute carrier family 28 member 2 Protein, solute carrier family 28 (concentrative nucleoside transporter), member 2 L homeolog Protein, sodium/nucleoside cotransporter 2 Protein, sodium/nucleoside cotransporter 1-like Protein, solute carrier family 28 (sodium-coupled nucleoside transporter), member 2 Protein, SLC28A2 Protein, slc28a2.L Protein, LOC100013132 Protein, LOC100226446 Protein, Slc28a2 Protein
- Sujet
- Sodium/nucleoside cotransporter 2 (Concentrative nucleoside transporter 2) (CNT 2) (hCNT2) (Na(+)/nucleoside cotransporter 2) (Sodium-coupled nucleoside transporter 2) (Sodium/purine nucleoside co-transporter) (SPNT) (Solute carrier family 28 member 2),FUNCTION: Sodium-dependent and purine-selective transporter (PubMed:9435697, PubMed:10087507). Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine) (PubMed:9435697, PubMed:10087507, PubMed:21795683). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues (PubMed:9435697). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). {ECO:0000269|PubMed:10087507, ECO:0000269|PubMed:21795683, ECO:0000269|PubMed:9435697, ECO:0000305|PubMed:35307651}.
- Poids moléculaire
- 71.9 kDa
- UniProt
- O43868
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