SLC2A3 Protein (AA 1-496) (Strep Tag)
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- Antigène Voir toutes SLC2A3 Protéines
- SLC2A3 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 3 (SLC2A3))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-496
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Origine
- Humain
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette SLC2A3 protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Séquence
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MGTQKVTPAL IFAITVATIG SFQFGYNTGV INAPEKIIKE FINKTLTDKG NAPPSEVLLT SLWSLSVAIF SVGGMIGSFS VGLFVNRFGR RNSMLIVNLL AVTGGCFMGL CKVAKSVEML ILGRLVIGLF CGLCTGFVPM YIGEISPTAL RGAFGTLNQL GIVVGILVAQ IFGLEFILGS EELWPLLLGF TILPAILQSA ALPFCPESPR FLLINRKEEE NAKQILQRLW GTQDVSQDIQ EMKDESARMS QEKQVTVLEL FRVSSYRQPI IISIVLQLSQ QLSGINAVFY YSTGIFKDAG VQEPIYATIG AGVVNTIFTV VSLFLVERAG RRTLHMIGLG GMAFCSTLMT VSLLLKDNYN GMSFVCIGAI LVFVAFFEIG PGPIPWFIVA ELFSQGPRPA AMAVAGCSNW TSNFLVGLLF PSAAHYLGAY VFIIFTGFLI TFLAFTFFKV PETRGRTFED ITRAFEGQAH GADRSGKDGV MEMNSIEPAK ETTTNV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product SLC2A3 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- SLC2A3 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 3 (SLC2A3))
- Autre désignation
- SLC2A3 (SLC2A3 Produits)
- Synonymes
- slc2a3 Protein, wu:fb92c06 Protein, zgc:92476 Protein, SLC2A3 Protein, GLUT3 Protein, AA408729 Protein, AL023014 Protein, AL024341 Protein, AU040424 Protein, C78366 Protein, Glut-3 Protein, Glut3 Protein, CEF-GT3 Protein, GLUT-3 Protein, SLC2A14 Protein, solute carrier family 2 (facilitated glucose transporter), member 3a Protein, solute carrier family 2 member 3 Protein, solute carrier family 2 (facilitated glucose transporter), member 3 Protein, solute carrier family 2 member 14 Protein, solute carrier family 2, facilitated glucose transporter member 3 Protein, slc2a3a Protein, SLC2A3 Protein, Slc2a3 Protein, SLC2A14 Protein, LOC100125981 Protein
- Sujet
- Solute carrier family 2, facilitated glucose transporter member 3 (Glucose transporter type 3, brain) (GLUT-3),FUNCTION: Facilitative glucose transporter (PubMed:9477959, PubMed:26176916). Can also mediate the uptake of various other monosaccharides across the cell membrane (PubMed:9477959, PubMed:26176916). Mediates the uptake of glucose, 2-deoxyglucose, galactose, mannose, xylose and fucose, and probably also dehydroascorbate (PubMed:9477959, PubMed:26176916). Does not mediate fructose transport (PubMed:9477959, PubMed:26176916). Required for mesendoderm differentiation (By similarity). {ECO:0000250|UniProtKB:P32037, ECO:0000269|PubMed:26176916, ECO:0000269|PubMed:8457197, ECO:0000269|PubMed:9477959}.
- Poids moléculaire
- 53.9 kDa
- UniProt
- P11169
- Pathways
- L'effet Warburg
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