PTPRU Protein (AA 19-1446) (rho-1D4 tag)
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- Antigène Voir toutes PTPRU Protéines
- PTPRU (Protein Tyrosine Phosphatase, Receptor Type, U (PTPRU))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 19-1446
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Origine
- Humain
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette PTPRU protéine est marqué à la rho-1D4 tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Séquence
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ETETPAAGCT FEEASDPAVP CEYSQAQYDD FQWEQVRIHP GTRAPADLPH GSYLMVNTSQ HAPGQRAHVI FQSLSENDTH CVQFSYFLYS RDGHSPGTLG VYVRVNGGPL GSAVWNMTGS HGRQWHQAEL AVSTFWPNEY QVLFEALISP DRRGYMGLDD ILLLSYPCAK APHFSRLGDV EVNAGQNASF QCMAAGRAAE AERFLLQRQS GALVPAAGVR HISHRRFLAT FPLAAVSRAE QDLYRCVSQA PRGAGVSNFA ELIVKEPPTP IAPPQLLRAG PTYLIIQLNT NSIIGDGPIV RKEIEYRMAR GPWAEVHAVS LQTYKLWHLD PDTEYEISVL LTRPGDGGTG RPGPPLISRT KCAEPMRAPK GLAFAEIQAR QLTLQWEPLG YNVTRCHTYT VSLCYHYTLG SSHNQTIREC VKTEQGVSRY TIKNLLPYRN VHVRLVLTNP EGRKEGKEVT FQTDEDVPSG IAAESLTFTP LEDMIFLKWE EPQEPNGLIT QYEISYQSIE SSDPAVNVPG PRRTISKLRN ETYHVFSNLH PGTTYLFSVR ARTGKGFGQA ALTEITTNIS APSFDYADMP SPLGESENTI TVLLRPAQGR GAPISVYQVI VEEERARRLR REPGGQDCFP VPLTFEAALA RGLVHYFGAE LAASSLPEAM PFTVGDNQTY RGFWNPPLEP RKAYLIYFQA ASHLKGETRL NCIRIARKAA CKESKRPLEV SQRSEEMGLI LGICAGGLAV LILLLGAIIV IIRKGRDHYA YSYYPKPVNM TKATVNYRQE KTHMMSAVDR SFTDQSTLQE DERLGLSFMD THGYSTRGDQ RSGGVTEASS LLGGSPRRPC GRKGSPYHTG QLHPAVRVAD LLQHINQMKT AEGYGFKQEY ESFFEGWDAT KKKDKVKGSR QEPMPAYDRH RVKLHPMLGD PNADYINANY IDGYHRSNHF IATQGPKPEM VYDFWRMVWQ EHCSSIVMIT KLVEVGRVKC SRYWPEDSDT YGDIKIMLVK TETLAEYVVR TFALERRGYS ARHEVRQFHF TAWPEHGVPY HATGLLAFIR RVKASTPPDA GPIVIHCSAG TGRTGCYIVL DVMLDMAECE GVVDIYNCVK TLCSRRVNMI QTEEQYIFIH DAILEACLCG ETTIPVSEFK ATYKEMIRID PQSNSSQLRE EFQTLNSVTP PLDVEECSIA LLPRNRDKNR SMDVLPPDRC LPFLISTDGD SNNYINAALT DSYTRSAAFI VTLHPLQSTT PDFWRLVYDY GCTSIVMLNQ LNQSNSAWPC LQYWPEPGRQ QYGLMEVEFM SGTADEDLVA RVFRVQNISR LQEGHLLVRH FQFLRWSAYR DTPDSKKAFL HLLAEVDKWQ AESGDGRTIV HCLNGGGRSG TFCACATVLE MIRCHNLVDV FFAAKTLRNY KPNMVETMDQ YHFCYDVALE YLEGLESR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human PTPRU Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin-free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product PTPRU Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- PTPRU (Protein Tyrosine Phosphatase, Receptor Type, U (PTPRU))
- Autre désignation
- PTPRU (PTPRU Produits)
- Synonymes
- FMI Protein, PCP-2 Protein, PTP Protein, PTP-J Protein, PTP-PI Protein, PTP-RO Protein, PTPPSI Protein, PTPRO Protein, PTPU2 Protein, R-PTP-PSI Protein, R-PTP-U Protein, hPTP-J Protein, Ftp-1 Protein, PTP-lambda Protein, PTPlambda Protein, Pcp2 Protein, Ptpf Protein, Ptprl Protein, R-PTP-psi Protein, RPTPlambda Protein, RPTP-psi Protein, fj42a04 Protein, fk66g06 Protein, ptpru Protein, wu:fj42a04 Protein, wu:fk66g06 Protein, protein tyrosine phosphatase, receptor type U Protein, protein tyrosine phosphatase, receptor type, U Protein, protein tyrosine phosphatase, receptor type, U, b Protein, PTPRU Protein, Ptpru Protein, ptprub Protein
- Sujet
- Tyrosine-protein phosphatase which dephosphorylates CTNNB1. Regulates CTNNB1 function both in cell adhesion and signaling. May function in cell proliferation and migration and play a role in the maintenance of epithelial integrity. May play a role in megakaryocytopoiesis. {ECO:0000269|PubMed:10397721, ECO:0000269|PubMed:12501215, ECO:0000269|PubMed:16574648}.
- Poids moléculaire
- 161.7 kDa Including tag.
- UniProt
- Q92729
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