ATOH7 Protein (AA 1-149) (Strep Tag)
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- Antigène Voir toutes ATOH7 Protéines
- ATOH7 (Atonal Homolog 7 (ATOH7))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-149
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ATOH7 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MKSACKPHGP PAGARGAPPC AGAAERAVSC AGPGRLESAA RRRLAANARE RRRMQGLNTA FDRLRRVVPQ WGQDKKLSKY ETLQMALSYI IALTRILAEA ERDWVGLRCE QRGRDHPYLP FPGARLQVDP EPYGQRLFGF QPEPFPMAS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product ATOH7 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ATOH7 (Atonal Homolog 7 (ATOH7))
- Autre désignation
- Atoh7 (ATOH7 Produits)
- Synonymes
- Math5 Protein, bHLHa13 Protein, ATH5 Protein, Xath5 Protein, Xath5a Protein, Xath5b Protein, ath5 Protein, atoh5a-A Protein, math5 Protein, zath5 Protein, ATOH7 Protein, atoh5b-A Protein, atoh7 Protein, atonal bHLH transcription factor 7 Protein, atonal bHLH transcription factor 7 S homeolog Protein, atonal bHLH transcription factor 7 L homeolog Protein, ATOH7 Protein, atoh7.S Protein, Atoh7 Protein, atoh7 Protein, atoh7.L Protein
- Sujet
- Transcription factor Atoh7 (Atonal bHLH transcription factor 7) (Helix-loop-helix protein mATH-5) (mATH5) (Protein atonal homolog 7),FUNCTION: Transcription factor that binds to DNA at the consensus sequence 5'-CAG[GC]TG-3' (PubMed:33712461). Dimerization with TCF3 isoform E47 may be required in certain situations (By similarity). Binds to gene promoters and enhancer elements, and thereby regulates a transcriptional program of retinal ganglion cell (RGC) determinant genes (PubMed:33712461). Although the exact mechanism is not certain, retinal transcription regulation by ATOH7 has a role in RGC determination and survival, photoreceptor population development, targeting of RGC axons to the optic nerve and development of the retino-hypothalamic tract (PubMed:11493566, PubMed:11156601, PubMed:12451142, PubMed:33712461). Binds to its own promoter and enhancer sequences, suggesting autoregulation of ATOH7 transcription (PubMed:33712461). Required for retinal circadian rhythm photoentrainment (PubMed:12451142, PubMed:26392540). Plays a role in brainstem auditory signaling and binaural processing (PubMed:17977745). {ECO:0000250|UniProtKB:Q8N100, ECO:0000269|PubMed:11156601, ECO:0000269|PubMed:11493566, ECO:0000269|PubMed:12451142, ECO:0000269|PubMed:17977745, ECO:0000269|PubMed:26392540, ECO:0000269|PubMed:33712461}.
- Poids moléculaire
- 16.6 kDa
- UniProt
- Q9Z2E5
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