ACSBG2 Protein (AA 1-667) (Strep Tag)
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- Antigène Voir toutes ACSBG2 Protéines
- ACSBG2 (Acyl-CoA Synthetase Bubblegum Family Member 2 (ACSBG2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-667
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ACSBG2 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MTQEKKAEDL ERGTDATSAV PRLWSTHCDG EVLLRLSKHG PGHETPMTIP ELFQESAERF SAYPALASKN GKKWDTLTFS QYYEMCRKAA KSLIKLGLQR FQCVGILGFN SVEWVVTALG TILAGGLCVG IYATNSAEAC QYVIQQANVS ILIVENDQQL QKILLIPPDK METVKAIVQY KLPLMESMAN LYSWNDFMEL GNDIPNIQLD RVILSQKANQ CAVILYTSGT TGTPKGVLLS HDNITWTAGA MSQEMEINRV SGKQNTIVSY LPLSHIAAQL TDIWIPIKIG ALTFFAQPDA LRGTLVYTLQ EVKPTLFMGV PRIWEKMQDT IKENVARSSR LRKKAFAWAK MLGLKVNTKR MLGKRDIPMN YRMAKALVFA KVRTSLGLDN CHAFFSSASP LSQDVSEFFL SLDIPIGEIY GMSECSGPHT VSNKSVYRVL SCGKVLSGCK NMLYNQNKEG VGEVCMWGRH VFMGYLNKEE ATLEALDENG WLHSGDIGRL DSHDFLYITG RIKEILITAG GENVSPIPIE TLVKEKIPII SHAMLVGDKA KFLCMLLTLK CETDRKSGEP LNKLSVEAKS FCQMLGSQAT TVSDILKSRD QVVYTAIQYG IDIVNQQAMS DSHRIRKWII LEKDFSIQGG ELGPTSKLKR SVITQKYKAQ IDSMYLS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ACSBG2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ACSBG2 (Acyl-CoA Synthetase Bubblegum Family Member 2 (ACSBG2))
- Autre désignation
- Acsbg2 (ACSBG2 Produits)
- Synonymes
- ACSBG2 Protein, fk81d02 Protein, im:7046047 Protein, sb:cb76 Protein, si:dkey-240a9.3 Protein, wu:fj55d04 Protein, wu:fk81d02 Protein, BGR Protein, BRGL Protein, PRTD-NY3 Protein, PRTDNY3 Protein, Bgr Protein, acyl-CoA synthetase bubblegum family member 2 Protein, long-chain-fatty-acid--CoA ligase ACSBG2 Protein, acyl-CoA synthetase bubblegum family member 2 L homeolog Protein, ACSBG2 Protein, LOC476732 Protein, acsbg2 Protein, acsbg2.L Protein, Acsbg2 Protein
- Sujet
- Long-chain-fatty-acid--CoA ligase ACSBG2 (EC 6.2.1.3) (Acyl-CoA synthetase bubblegum family member 2) (Arachidonate--CoA ligase ACSBG2) (EC 6.2.1.15) (Bubblegum-related protein),FUNCTION: Catalyzes the conversion of fatty acids such as long chain and very long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Can activate diverse saturated, monosaturated and polyunsaturated fatty acids. Has increased ability to activate oleic and linoleic acid. May play a role in spermatogenesis. {ECO:0000250|UniProtKB:Q5FVE4}.
- Poids moléculaire
- 74.3 kDa
- UniProt
- Q2XU92
- Pathways
- Monocarboxylic Acid Catabolic Process
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