DHPS Protein (AA 1-369) (Strep Tag)
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- Antigène Voir toutes DHPS Protéines
- DHPS (Deoxyhypusine Synthase (DHPS))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-369
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette DHPS protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Séquence
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MEGTPPGAAP SSALAAVLKH SSALPPESAQ VQGYDFNRGV DYHALLDAYR TTGFQATNFG RAVQQVNAMI EKKLEPLAVD EDHHADLTQS RRPLTGCTIF LGYTSNLISS GIRETIRYLV QHNMVDVLVT TAGGVEEDLI KCLAPTYLGE FSLRGKELRE SGINRIGNLL VPNDNYCKFE DWLMPILDQM VLEQNTEGVK WTPSKMISRL GKEINNPDSV YYWAHKNHIP VLSPALTDGS LGDMIFFHSY KNPGLVLDIV EDLRLINTQA IFAKRSGMII LGGGVVKHHI ANANLMRNGA DYAVYINTAQ EFDGSDSGAR PDEAVSWGKI RMDAQPVKVY ADASLVFPLL VAETFAQKAD AFRAEKNED
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product DHPS Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- DHPS (Deoxyhypusine Synthase (DHPS))
- Autre désignation
- Dhps (DHPS Produits)
- Synonymes
- MGC76269 Protein, zgc:56018 Protein, zgc:77325 Protein, wu:fb52g04 Protein, wu:fj48f12 Protein, zgc:111900 Protein, MGC82178 Protein, DDBDRAFT_0186679 Protein, DDBDRAFT_0266388 Protein, DDB_0186679 Protein, DDB_0266388 Protein, DHS Protein, DS Protein, MIG13 Protein, Dhs Protein, DYS Protein, EDA22 Protein, K18J17.7 Protein, K18J17_7 Protein, deoxyhypusine synthase Protein, embryo sac development arrest 22 Protein, deoxyhypusine synthase Protein, deoxyhypusine synthase S homeolog Protein, Deoxyhypusine synthase Protein, DHPS Protein, dhps Protein, dhps.S Protein, SHELL_RS05250 Protein, PF14_0125 Protein, CNJ02480 Protein, Tb10.70.4900 Protein, Tb927.1.870 Protein, MMAH_RS09430 Protein, TAGG_RS03310 Protein, TAGG_RS05175 Protein, Mesil_2448 Protein, Trad_0088 Protein, Igag_1842 Protein, Desac_0724 Protein, MZHIL_RS05480 Protein, MZHIL_RS05835 Protein, MZHIL_RS06250 Protein, Dhps Protein, DHS Protein
- Sujet
- Deoxyhypusine synthase (DHS) (EC 2.5.1.46),FUNCTION: Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a critical lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue. This is the first step of the post-translational modification of that lysine into an unusual amino acid residue named hypusine. Hypusination is unique to mature eIF-5A factor and is essential for its function. {ECO:0000250|UniProtKB:P49366}.
- Poids moléculaire
- 40.6 kDa
- UniProt
- Q3TXU5
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