IRGM Protein (AA 1-409) (Strep Tag)
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- Antigène Voir toutes IRGM Protéines
- IRGM (Immunity-Related GTPase Family, M (IRGM))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-409
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette IRGM protéine est marqué à la Strep Tag.
- Application
- SDS-PAGE (SDS), ELISA, Western Blotting (WB)
- Séquence
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MKPSHSSCEA APLLPNMAET HYAPLSSAFP FVTSYQTGSS RLPEVSRSTE RALREGKLLE LVYGIKETVA TLSQIPVSIF VTGDSGNGMS SFINALRVIG HDEDASAPTG VVRTTKTRTE YSSSHFPNVV LWDLPGLGAT AQTVEDYVEE MKFSTCDLFI IIASEQFSSN HVKLSKIIQS MGKRFYIVWT KLDRDLSTSV LSEVRLLQNI QENIRENLQK EKVKYPPVFL VSSLDPLLYD FPKLRDTLHK DLSNIRCCEP LKTLYGTYEK IVGDKVAVWK QRIANESLKN SLGVRDDDNM GECLKVYRLI FGVDDESVQQ VAQSMGTVVM EYKDNMKSQN FYTLRREDWK LRLMTCAIVN AFFRLLRFLP CVCCCLRRLR HKRMLFLVAQ DTKNILEKIL RDSIFPPQI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product IRGM Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- IRGM (Immunity-Related GTPase Family, M (IRGM))
- Autre désignation
- Irgm1 (IRGM Produits)
- Synonymes
- Ifi1 Protein, Iigp3 Protein, Iipg3 Protein, Irgm Protein, LRG-47 Protein, IFI1 Protein, IRGM1 Protein, LRG47 Protein, immunity-related GTPase family M member 1 Protein, immunity-related GTPase M Protein, immunity related GTPase M Protein, immunity-related GTPase family M protein Protein, Irgm1 Protein, Irgm Protein, IRGM Protein, LOC481473 Protein
- Sujet
- Immunity-related GTPase family M protein 1 (EC 3.6.5.-) (Interferon-inducible GTPase 3) (Interferon-inducible protein 1) (LPS-stimulated RAW 264.7 macrophage protein 47) (LRG-47),FUNCTION: Immunity-related GTPase that plays important roles in innate immunity and inflammatory response (PubMed:11457893, PubMed:14576437, PubMed:14707092, PubMed:15908352, PubMed:16339555, PubMed:17911638, PubMed:17982087, PubMed:19620982, PubMed:19920210). Acts as a dynamin-like protein that binds to intracellular membranes and promotes remodeling and trafficking of those membranes (PubMed:19620982, PubMed:27098192). Required for clearance of acute protozoan and bacterial infections by interacting with autophagy and lysosome regulatory proteins, thereby promoting the fusion of phagosomes with lysosomes for efficient degradation of cargo including microbes (PubMed:11457893, PubMed:14576437, PubMed:15607973, PubMed:14707092, PubMed:15908352, PubMed:16339555, PubMed:17982087, PubMed:19620982, PubMed:19920210, PubMed:24751652, PubMed:21757726, PubMed:22874556, PubMed:32453761). Regulates selective autophagy, including xenophagy and mitophagy, both directly and indirectly (PubMed:15607973, PubMed:21757726). Directly regulates autophagy by acting as a molecular adapter that promotes the coassembly of the core autophagy machinery to mediate antimicrobial defense: Irgm1 (1) activates AMPK, which in turn phosphorylates ULK1 and BECN1 to induce autophagy, (2) promotes the coassembly of ULK1 and BECN1, enhancing BECN1-interacting partners and (3) influences the composition of the BECN1 complex, by competing with the negative regulators BCL2 and RUBCN, to trigger autophagy (By similarity). Also activates autophagy by promoting recruitment of STX17 to autophagosomes (By similarity). In collaboration with ATG8 proteins, regulate lysosomal biogenesis, a fundamental process for any autophagic pathway, by promoting TFEB dephosphorylation (By similarity). Also modulates autophagy by assisting with autophagosome formation and preventing lysosomal deacidification (PubMed:21757726). Regulates autophagy by affecting mitochondrial fusion and fission (PubMed:24751652). Also involved in M1 macrophage activation for the production of proinflammatory cytokines (PubMed:15908352, PubMed:27439214, PubMed:27443879). While activating autophagy, acts as a key negative regulator of the inflammatory and interferon responses both by (1) promoting mitophagy and (2) mediating autophagy-dependent degradation of effectors of the inflammatory response (PubMed:30612879, PubMed:34467632, PubMed:33510463). Promotes degradation of damaged and IFNG/IFN-gamma-stressed mitochondria via mitophagy, preventing cytosolic release of ligands that activate inflammation (PubMed:32715615, PubMed:33510463). Negatively regulates interferon-signaling in hematopoietic stem cells, preserving hematopoietic stem cell number and function (PubMed:18371424, PubMed:21633090). Promotes expansion of activated CD4(+) T-cells by inhibiting IFNG/IFN-gamma signaling, thereby preventing Ifng-mediated cell death of CD4(+) T-cells (PubMed:18806793). Acts as a suppressor of inflammation by promoting recruitment of inflammation effectors, such as CGAS, RIGI/RIG-I and NLRP3, to autophagosome membranes, leading to their SQSTM1/p62-dependent autophagic degradation (By similarity). Also directly inhibits assembly of the NLRP3 inflammasome by preventing the association between NLRP3 and PYCARD (By similarity). Acts as a negative regulator of antiviral innate immune response by suppressing the RIPK2-dependent pro-inflammatory response: mediates recruitment of RIPosomes, composed of RIPK2 and NOD1 or NOD2, to autophagosome membranes, promoting their SQSTM1/p62-dependent autophagic degradation (By similarity). {ECO:0000250|UniProtKB:A1A4Y4, ECO:0000269|PubMed:11457893, ECO:0000269|PubMed:14576437, ECO:0000269|PubMed:14707092, ECO:0000269|PubMed:15607973, ECO:0000269|PubMed:15908352, ECO:0000269|PubMed:16339555, ECO:0000269|PubMed:17911638, ECO:0000269|PubMed:17982087, ECO:0000269|PubMed:18371424, ECO:0000269|PubMed:18806793, ECO:0000269|PubMed:19620982, ECO:0000269|PubMed:19920210, ECO:0000269|PubMed:21633090, ECO:0000269|PubMed:21757726, ECO:0000269|PubMed:22874556, ECO:0000269|PubMed:24751652, ECO:0000269|PubMed:27098192, ECO:0000269|PubMed:27439214, ECO:0000269|PubMed:27443879, ECO:0000269|PubMed:30612879, ECO:0000269|PubMed:32453761, ECO:0000269|PubMed:32715615, ECO:0000269|PubMed:33510463, ECO:0000269|PubMed:34467632}.
- Poids moléculaire
- 46.6 kDa
- UniProt
- Q60766
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