HSPA4 Protein (AA 1-841) (Strep Tag)
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- Antigène Voir toutes HSPA4 Protéines
- HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-841
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette HSPA4 protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Séquence
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MSVVGIDLGF QSCYVAVARA GGIETIANEY SDRCTPACVS FGPKNRSIGA AAKSQVISNA KNTVQGFKRF HGRAFSDPFV EAEKSNLAYD IVQLPTGLTG IKVTYMEEER NFTTEQVTAM LLSKLKETAE SVLKKPVVDC VVSVPSFYTD AERRSVMDAT QIAGLNCLRL MNETTAVALA YGIYKQDLPA LEEKPRNVVF VDMGHSAYQV SVCAFNKGKL KVLATAFDTT LGGRKFDEVL VNHFCEEFGK KYKLDIKSKI RALLRLSQEC EKLKKLMSAN ASDLPLSIEC FMNDIDVSGT MNRGKFLEMC DDLLARVEPP LRSVLEQSKL KKEDIYAVEI VGGATRIPAV KEKISKFFGK ELSTTLNADE AVTRGCALQC AILSPAFKVR EFSITDVVPY PISLRWNSPA EEGLSDCEVF PKNHAAPFSK VLTFYRKEPF TLEAYYSSPQ DLPYPDPAIA QFSVQKVTPQ SDGSSSKVKV KVRVNVHGIF SVSSAALVEV HKSEESEEPM ETDQNAKEEE KMQVDQEEPH TEEQQQQPQT PAENKAESEE METSQAGSKD KKTDQPPQAK KAKVKTSTVD LPIEHTLWQL DREMLALYTE NEGKMIMQDK LEKERNDAKN AVEEYVYEMR DKLSGEYEKF VSEDDRNTFT LKLEDTENWL YEDGEDQPKQ VYVDKLAELK SLGQPIKTRF QESEERPKLF EELGKQIQQY MKVISSFKNK EDQYEHLDAA DVTKVEKSTN EAMEWMNSKL NLQNKQSLTV DPVVKTKEIE AKIKELTSIC SPIISKPKPK VEPPKEEPKH AEQNGPVDGQ GDNPGSQAAE HGADTAVPSD GDKKLPEMDI D
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product HSPA4 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- HSPA4 (Heat Shock 70kDa Protein 4 (HSPA4))
- Autre désignation
- Hspa4 (HSPA4 Produits)
- Synonymes
- APG-2 Protein, HS24/P52 Protein, HSPH2 Protein, RY Protein, hsp70 Protein, hsp70RY Protein, Hsp110 Protein, Hsp70 Protein, irp94 Protein, 70kDa Protein, AI317151 Protein, Hsp70RY Protein, mKIAA4025 Protein, hspa4 Protein, wu:fi30e11 Protein, zgc:55743 Protein, zgc:77413 Protein, hs24/p52 Protein, hspa4-a Protein, osp94 Protein, pg-2 Protein, hspa4l Protein, wu:fc41d05 Protein, wu:fi59h02 Protein, wu:fj35c08 Protein, zgc:55506 Protein, heat shock protein family A (Hsp70) member 4 Protein, heat shock protein family A member 4 Protein, heat shock protein 4 Protein, heat shock protein 4b Protein, heat shock protein family A (Hsp70) member 4 S homeolog Protein, heat shock protein 4a Protein, HSPA4 Protein, Hspa4 Protein, hspa4b Protein, hspa4.S Protein, hspa4a Protein
- Sujet
- Heat shock 70 kDa protein 4 (Heat shock 70-related protein APG-2)
- Poids moléculaire
- 94.1 kDa
- UniProt
- Q61316
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