PHF15 Protein (AA 1-829) (Strep Tag)
-
- Antigène Voir toutes PHF15 Protéines
- PHF15 (PHD Finger Protein 15 (PHF15))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-829
- Origine
- Souris
-
Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette PHF15 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
-
MEEKRRKYSI SSDNSDTTDG HVTSTSASRC SKLPSSTKSG WPRQNEKKPS EVFRTDLITA MKIPDSYQLS PDDYYILADP WRQEWEKGVQ VPAGAEAIPE PVVRLLPPLK GPPTQMSPDS PTLGEGAHPD WPGGSRYDLD EIDAYWLELL NSELKEMEKP ELDELTLERV LEELETLCHQ NMAQAIETQE GLGIEYDEDV VCDVCRSPEG EDGNEMVFCD KCNVCVHQAC YGILKVPTGS WLCRTCALGV QPKCLLCPKR GGALKPTRSG TKWVHVSCAL WIPEVSIGCP EKMEPITKIS HIPASRWALS CSLCKECTGT CIQCSMPSCI TAFHVTCAFD RGLEMRTILA DNDEVKFKSL CQEHSDGGPR SEPTSEPVEP SQAVEDLEKV TLRKQRLQQL EENFYELVEP AEVAERLDLA EALVDFIYQY WKLKRRANAN QPLLTPKTDE VDNLAQQEQD VLYRRLKLFT HLRQDLERVR NLCYMVTRRE RTKHTICKLQ EQIFHLQMKL IEQDLCREPS GRRSKGKKND SKRKGREGPK GSSPEKKEKV KAGPESVLGQ LGLSTSFPID GTFFNSWLAQ SVQITAEDMA MSEWSLNSGH REDPAPGLLS EELLQDEETL LSFMRDPSLR PGDPARKARG RTRLPAKKKP SPLQDGPSAR TTPDKQPKKA WAQDGKGTQG PPMRKPPRRT SSHLPSSPAA GDCPVPATLE SPPPLASEIL DKTAPMASDL NVQVPGPTVS PKPLGRLRPP REMKVSRKSP GARSDAGTGL PSAVAERPKV SLHFDTEADG YFSDEEMSDS EVEAEDSGVQ RASREAGAEE VVRMGVLAS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product PHF15 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- PHF15 (PHD Finger Protein 15 (PHF15))
- Autre désignation
- Jade2 (PHF15 Produits)
- Synonymes
- PHF15 Protein, JADE2 Protein, 1200017K05Rik Protein, AI480685 Protein, Jade2 Protein, mKIAA0239 Protein, jade family PHD finger 2 Protein, jade family PHD finger 2 L homeolog Protein, PHD finger protein 15 Protein, Jade2 Protein, JADE2 Protein, jade2.L Protein, GL50803_15559 Protein, jade2 Protein
- Sujet
- E3 ubiquitin-protein ligase Jade-2 (EC 2.3.2.27) (Jade family PHD finger protein 2) (PHD finger protein 15),FUNCTION: Scaffold subunit of some HBO1 complexes, which have a histone H4 acetyltransferase activity (By similarity). Acts as a E3 ubiquitin-protein ligase mediating the ubiquitination and subsequent proteasomal degradation of target protein histone demethylase KDM1A (PubMed:25018020). Also acts as a ubiquitin ligase E3 toward itself (PubMed:25018020). Positive regulator of neurogenesis (PubMed:25018020). {ECO:0000250|UniProtKB:Q9NQC1, ECO:0000269|PubMed:25018020}.
- Poids moléculaire
- 92.2 kDa
- UniProt
- Q6ZQF7
-