ENKD1 Protein (AA 1-346) (Strep Tag)
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- Antigène Tous les produits ENKD1
- ENKD1 (Enkurin Domain Containing 1 (ENKD1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-346
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ENKD1 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MCEGPSRISG PIPPDPTLCP DYYRRPASAQ GRLEGNALKL DLLTSGRDLD SSPPRGPRIR PEAREILERG QRGVGDVLLQ LGCISLGSGV SPKRKNPKDH EKENLRRIKE IQRRFQDQER SREQGQPKPL KALWRSPKYD NVESRVKARM KELGPTSVTE PAHFLRAHSR CGPGLPPSRA SSPQLALPGP QAKGPGLGVD FISRNALAAK RAPRRHSRSL QVLAQVQEQQ RQAQERYNAT QKGHVPHYLL ERRDLWRKEA EARQRSQPDP SMPPGHTLMP ENQRLETLNN LLQSQSQLLR ELVLLPAGAD SLRAQGHRAE LDRKLVQIEE AIKIFSRPKV FVKMDT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ENKD1 (Enkurin Domain Containing 1 (ENKD1))
- Autre désignation
- Enkd1 (ENKD1 Produits)
- Synonymes
- C16orf48 Protein, DAKV6410 Protein, C18H16orf48 Protein, AI606951 Protein, E130303B06Rik Protein, enkurin domain containing 1 Protein, ENKD1 Protein, Enkd1 Protein
- Sujet
- Enkurin domain-containing protein 1,FUNCTION: Microtubule-binding protein which regulates microtubule organization and stability (By similarity). Promotes the stability of astral microtubules and facilitates the proper orientation of the mitotic spindle (By similarity). This allows the oriented division of basal keratinocytes and contributes to epidermal stratification (PubMed:35197565). Required for the assembly of both primary and motile cilia (PubMed:35301795, PubMed:35072334). Destabilizes the interaction between CCP110 and CEP97 by competing with CEP97 for binding to CCP110 which promotes the removal of CCP110 and CEP97 from the mother centriole and allows the initiation of ciliogenesis (PubMed:35301795). {ECO:0000250|UniProtKB:Q9H0I2, ECO:0000269|PubMed:35072334, ECO:0000269|PubMed:35197565, ECO:0000269|PubMed:35301795}.
- Poids moléculaire
- 39.0 kDa
- UniProt
- Q7TSV9
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