UTP25/DIEXF Protein (AA 1-772) (Strep Tag)
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- Antigène Voir toutes UTP25/DIEXF (DIEXF) Protéines
- UTP25/DIEXF (DIEXF) (Digestive Organ Expansion Factor Homolog (DIEXF))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-772
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette UTP25/DIEXF protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MGKRRNRGRS QMLSTMTKKQ KKHLRDFGEE HPFYDRVSKK EAKPQICQLP ESSDSSHSES ESESEQEHVS GYHRLLATLK NVSEEEEEEE EEEEEEEEEE EEEEEEEEDD SAVGDAEMNE EAGSEDGSVG EAAVSEAAEE AAETQEHMSL ADNSKEKDGE EPPGVSQKSS EEFTDVKHES LFSLETNFLE EDSGGSCSQR PSQDPFQQHV NKELKEKEIQ AAASSPPATQ QLKWPVLGHL VFSSKFQKTE TFKPPKDIDL KLLHLQKPLE STWAKTNSQF LSGPQKSNSS FTPLQKELFL IMNSYRDLFY PERTALKNGE EVRHVYCLHA INHVLKANAQ VLANNSRRRS QKLGVGEDDD FRDQGLTRPK VLIVVPFREA ALRVVQLFIS LLEGDSKKKI IVSNKKRFQG EYGSDPEERP PNLKRPEDYE AVFVGNIDDH FRIGVAILQR SIRLYAPFYS SDILIASPLG LRTIIGGEGE KKRDFDFLSS VELLIIDQAD IYLMQNWEHV LHLMNHMNLL PLDSHGVDFS RVRMWSLNNW SKYYRQTLLF GALQDAQINS VFNKHCINAQ GQVAVRNVPM TGSISHVLVQ LPHVFQRMEA QDLSSVIDAR FHFFINKILP QYRDAVMSHT LIYVPSYFDF VRLRNYFKKE ELNFTHICEY TQKSGISRAR HFFLQGEKQF LLLTERFHFY KRYTIKGIRN LIFYELPTYP HFYSEVCNML RATSRGEEAT WTCTVLYSKY DAQRLAAVVG VERAAQMLQS PKNVHLFVTG EK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- UTP25/DIEXF (DIEXF) (Digestive Organ Expansion Factor Homolog (DIEXF))
- Autre désignation
- Utp25 (DIEXF Produits)
- Synonymes
- C1orf107 Protein, DEF Protein, DJ434O14.5 Protein, UTP25 Protein, AA408296 Protein, AW045375 Protein, Def Protein, chunp6870 Protein, def Protein, dj434o14.5 Protein, dj434o14.5l Protein, fc51g08 Protein, fi05f05 Protein, wu:fc51g08 Protein, wu:fi05f05 Protein, digestive organ expansion factor homolog (zebrafish) Protein, digestive organ expansion factor homolog Protein, DIEXF Protein, Diexf Protein, diexf Protein
- Sujet
- U3 small nucleolar RNA-associated protein 25 homolog (Digestive organ expansion factor homolog) (UTP25 small subunit processor component),FUNCTION: Component of the ribosomal small subunit processome for the biogenesis of ribosomes, functions in pre-ribosomal RNA (pre-rRNA) processing (By similarity). Essential for embryonic development in part through the regulation of p53 pathway. Controls the expansion growth of digestive organs and liver (PubMed:29262616, PubMed:32303961). Also involved in the sympathetic neuronal development (By similarity). Mediates, with CAPN3, the proteasome-independent degradation of p53/TP53 (By similarity). {ECO:0000250|UniProtKB:Q68CQ4, ECO:0000250|UniProtKB:Q6PEH4, ECO:0000269|PubMed:29262616, ECO:0000269|PubMed:32303961}.
- Poids moléculaire
- 88.8 kDa
- UniProt
- Q8BTT6
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