PDXK Protein (AA 1-312) (Strep Tag)
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- Antigène Voir toutes PDXK Protéines
- PDXK (Pyridoxal Kinase (PDXK))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-312
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette PDXK protéine est marqué à la Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Séquence
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MEGECRVLSI QSHVVRGYVG NRAAMFPLQV LGFEVDAVNS VQFSNHTGYA HWKGQVLKSQ ELHELYEGLK VNDVNKYDYV LTGYTRDKSF LAMVVDIVRE LKQQNSRLVY VCDPVMGDKW NGEGSMYVPQ DLLPVYRDKV VPVADIITPN QFEAELLSGR KIHSQEEAFE VMDMLHCMGP DTVVITSSDL PSSQGSDYLI ALGSQRMRKP DGSTVTQRIR MEMRKVEAVF VGTGDLFAAM LLAWTHKHPD NLKVACEKTV SAMQHVLQRT IRCAKAEAGE GQKPSPAQLE LRMVQSKRDI EDPEIVVQAT VL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product PDXK Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- PDXK (Pyridoxal Kinase (PDXK))
- Autre désignation
- Pdxk (PDXK Produits)
- Synonymes
- C21orf124 Protein, C21orf97 Protein, PKH Protein, PNK Protein, 2310036D04Rik Protein, AA119688 Protein, C77999 Protein, Pdxk Protein, PSPTO5521 Protein, DDBDRAFT_0190079 Protein, DDBDRAFT_0191114 Protein, DDB_0190079 Protein, DDB_0191114 Protein, ATSOS4 Protein, K18L3.1 Protein, K18L3_1 Protein, SALT OVERLY SENSITIVE 4 Protein, pdxk Protein, si:dkey-263m10.2 Protein, si:dz69g10.1 Protein, cb1056 Protein, pdxkl Protein, si:dkey-281i8.2 Protein, wu:fa55a03 Protein, wu:fb10c12 Protein, zgc:101900 Protein, pyridoxal kinase Protein, pyridoxal (pyridoxine, vitamin B6) kinase Protein, Pyridoxal kinase Protein, pfkB-like carbohydrate kinase family protein Protein, pyridoxal (pyridoxine, vitamin B6) kinase b Protein, pyridoxal (pyridoxine, vitamin B6) kinase a Protein, PDXK Protein, Pdxk Protein, pdxY Protein, pdxK Protein, Bphyt_1541 Protein, pykA Protein, Kfla_4736 Protein, Kvar_1290 Protein, Mrub_2507 Protein, BC1002_1103 Protein, Fbal_1661 Protein, Lbys_0710 Protein, Intca_0131 Protein, Deima_3190 Protein, Mesop_1158 Protein, LOC100283562 Protein, pdxk Protein, SOS4 Protein, pdxkb Protein, pdxka Protein
- Sujet
- Pyridoxal kinase (EC 2.7.1.35) (Pyridoxine kinase),FUNCTION: Catalyzes the phosphorylation of the dietary vitamin B6 vitamers pyridoxal (PL), pyridoxine (PN) and pyridoxamine (PM) to form pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PNP) and pyridoxamine 5'-phosphate (PMP), respectively (By similarity). PLP is the active form of vitamin B6, and acts as a cofactor for over 140 different enzymatic reactions (By similarity). {ECO:0000250|UniProtKB:O00764}.
- Poids moléculaire
- 35.0 kDa
- UniProt
- Q8K183
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