TAF9 Protein (AA 1-264) (Strep Tag)
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- Antigène Voir toutes TAF9 Protéines
- TAF9 (RNA Polymerase II TBP-Associated Factor Subunit G (TAF9))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-264
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette TAF9 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MESGKMASPK SMPKDAQMMA QILKDMGITE YEPRVINQML EFAFRYVTTI LDDAKIYSSH AKKATVDADD VRLAIQCRAD QSFTSPPPRD FLLDIARQRN QTPLPLIKPY SGPRLPPDRY CLTAPNYRLK SLQKKAPAPA GRITVPRLSV GSVSSRPSTP TLGTPTPQTM SVSTKVGTPM SLTGQRFTVQ MPASQSPAVK ASIPATSTVQ NVLINPSLIG SKNILITTNM VSQNTAESAN ALKRKREDDD DDDDDDDDDD YDNM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product TAF9 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- TAF9 (RNA Polymerase II TBP-Associated Factor Subunit G (TAF9))
- Autre désignation
- Taf9 (TAF9 Produits)
- Synonymes
- AK6 Protein, CGI-137 Protein, CINAP Protein, CIP Protein, MGC:1603 Protein, MGC:3647 Protein, MGC:5067 Protein, TAF2G Protein, TAFII31 Protein, TAFII32 Protein, TAFIID32 Protein, hCINAP Protein, 2310012M09Rik Protein, 2810046E22Rik Protein, 4921516M08Rik Protein, AA673500 Protein, AU045423 Protein, Ak6 Protein, Taf2g Protein, im:7089279 Protein, wu:fb16h03 Protein, zgc:153410 Protein, TATA-box binding protein associated factor 9 Protein, adenylate kinase 6 L homeolog Protein, adenylate kinase isoenzyme 6-like Protein, SAGA complex/ transcription initiation factor Taf9 Protein, TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor Protein, TAF9 Protein, Taf9 Protein, ak6.L Protein, LOC100355082 Protein, taf9 Protein
- Sujet
- Transcription initiation factor TFIID subunit 9 (RNA polymerase II TBP-associated factor subunit G) (Transcription initiation factor TFIID 31 kDa subunit) (TAFII-31) (TAFII31) (Transcription initiation factor TFIID 32 kDa subunit) (TAFII-32) (TAFII32),FUNCTION: The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF9 is also a component of the TBP-free TAFII complex (TFTC), the PCAF histone acetylase complex and the STAGA transcription coactivator-HAT complex. TAF9 and its paralog TAF9B are involved in transcriptional activation as well as repression of distinct but overlapping sets of genes. Essential for cell viability. May have a role in gene regulation associated with apoptosis. {ECO:0000250|UniProtKB:Q16594}.
- Poids moléculaire
- 29.0 kDa
- UniProt
- Q8VI33
- Pathways
- Negative Regulation of intrinsic apoptotic Signaling
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