Mare/Nprl3/C16orf35 Protein (AA 1-569) (Strep Tag)
-
- Antigène Voir toutes Mare/Nprl3/C16orf35 (C16orf35) Protéines
- Mare/Nprl3/C16orf35 (C16orf35) (MARE (C16orf35))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-569
- Origine
- Souris
-
Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette Mare/Nprl3/C16orf35 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
-
MGDNTSPISV ILVSSGSRGN KLLFRYPFQR SQEHPASQTN KPRSRYAVNN TGEHADDQDG DSRFSDVILA TILATKSEMC GQKFELKIDN VRFVGHPTLL QHALGQVSKT DPSPKREAPT MILFNVVFAL RANADPSVIN CLHNLSRRIA TVLQHEERRC QYLTREAKLI LALQDEVSAM ADANEGPQSP FQHILPKCKL ARDLKEAYDS LCTSGVVRLH INSWLEVSFC LPHKIHYAAS SLIPPEAIER SLKAIRPYHA LLLLSDEKSL LSELPIDCSP ALVRVIKTTS AVKNLQQLAQ DADLALLQVF QLAAHLVYWG KAVIIYPLCE NNVYVMSPNA SVCLYSPLAE QFSRQFPSHD LPSVLAKFSL PVSLSEFRSP LAPPAQETQL IQMVVWMLQR RLLIQLHTYV CLMASPSEEE PRLREDDVPF TARVGGRSLS TPNALSFGSP TSSDDMTLTS PSMDNSSAEL LPSGDSPLNK RMTENLLASL SEHERAAILN VPAAQNPEDL RMFARLLHYF RGRHHLEEIM YNENTRRSQL LMLFDKFRSV LVVTTHEDPV IAVFQALLT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product C16orf35 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- Mare/Nprl3/C16orf35 (C16orf35) (MARE (C16orf35))
- Autre désignation
- Nprl3 (C16orf35 Produits)
- Synonymes
- C16orf35 Protein, CGTHBA Protein, HS-40 Protein, MARE Protein, NPR3 Protein, RMD11 Protein, Aag Protein, HS-26 Protein, Mare Protein, Phg Protein, Prox1 Protein, m(alpha)RE Protein, RGD1308665 Protein, c16orf35 Protein, cgthba Protein, hs-40 Protein, mare Protein, npr3 Protein, nprl3 Protein, rmd11 Protein, NPR3 like, GATOR1 complex subunit Protein, nitrogen permease regulator-like 3 Protein, NPR3-like, GATOR1 complex subunit Protein, NPR3-like, GATOR1 complex subunit L homeolog Protein, NPRL3 Protein, Nprl3 Protein, nprl3.L Protein
- Sujet
- GATOR1 complex protein NPRL3 (Nitrogen permease regulator 3-like protein),FUNCTION: As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway. In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway. {ECO:0000250|UniProtKB:Q12980}.
- Poids moléculaire
- 63.6 kDa
- UniProt
- Q8VIJ8
-