BCDIN3D Protein (AA 1-285) (Strep Tag)
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- Antigène Voir toutes BCDIN3D Protéines
- BCDIN3D (BCDIN3 Domain Containing (BCDIN3D))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-285
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette BCDIN3D protéine est marqué à la Strep Tag.
- Application
- SDS-PAGE (SDS), ELISA, Western Blotting (WB)
- Séquence
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MAADGTLSRG GVGEAVEEEH PGALEPGAAP FGNFPHYSRF HPPEQRLRLL PPELLRQLFP PEGPEKRPIL GLDVGCNSGD LSVALYKHFL SPRDGETCSG ASRELRILCC DIDPVLVERA ERDCPFPEAL TFITLDIMDQ ESRKVPLSSF LSQFGRSVFD MVFCMSVTMW IHLNHGDRGL CEFLAHVSSL CSYLLVEPQP WKCYRAAARR LRKLGLHSFD HFRSLAIRGD MAKQIVRILT QDHGMELACC FGNTSWDRSL LLFRAKHTHE TQAIPESSTK ETRTD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product BCDIN3D Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- BCDIN3D (BCDIN3 Domain Containing (BCDIN3D))
- Autre désignation
- Bcdin3d (BCDIN3D Produits)
- Synonymes
- 4930556P03Rik Protein, AV138748 Protein, BCDIN3 domain containing RNA methyltransferase Protein, BCDIN3 domain containing Protein, BCDIN3D Protein, Bcdin3d Protein
- Sujet
- RNA 5'-monophosphate methyltransferase (EC 2.1.1.-) (BCDIN3 domain-containing protein),FUNCTION: O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145, however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145. {ECO:0000250|UniProtKB:Q7Z5W3}.
- Poids moléculaire
- 32.0 kDa
- UniProt
- Q91YP1
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