NUF2 Protein (AA 1-463) (Strep Tag)
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- Antigène Voir toutes NUF2 Protéines
- NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-463
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette NUF2 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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METLSFPRYN VAELVVHIRN KLLTGADGKN LSKSDFLPNP KSDVLYMIYM KALQLVYGVR LEHFYMMPMN IEVTYPHLME GFLPVRSLFF YMDSFMPICR VNDFEIVDIL NPRTNRTSRF LSGIINFIHF RETCLEKCEE FLLQNKSSMV RMQQLSNVHQ EALMKLEKLN TVPAEEREEF KQFMDDIQEL QHLLNEEFRQ KTTLLQEEYA KMKSDISEKT KHLNEQKLSL VSLKEVEDNL KSKIVDSPEK LKNYKDKMKG TVQKLRSARE KVMEQYDIYR DSVDCLPSCQ LEVQLYQKKS QDLADNREKL SSLLKESLNL EDQIESDSSE LKKLKTEENS LIRMTTVKKE KLATARFKIN KKQEDVKHYK QAMIEDCNKV QEKRDAVCEQ VTTVNQEIHK IKSAIQQLRD TKKREILKSQ EIFVNLKSAL EKYHEGIEKV AEERSAKLEE KTAELKKRMV RMV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product NUF2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- NUF2 (NUF2, NDC80 Kinetochore Complex Component (NUF2))
- Autre désignation
- Nuf2 (NUF2 Produits)
- Synonymes
- 2410003C07Rik Protein, AU044112 Protein, C85691 Protein, Cdca1 Protein, NUF2R Protein, CDCA1 Protein, CT106 Protein, zgc:56708 Protein, cdca1 Protein, ct106 Protein, nuf2 Protein, nuf2-a Protein, nuf2-b Protein, nuf2r Protein, xNuf2 Protein, cdca1-B Protein, xnuf2 Protein, NUF2, NDC80 kinetochore complex component Protein, NUF2, NDC80 kinetochore complex component, homolog Protein, spindle pole body protein Nuf2 Protein, NUF2, NDC80 kinetochore complex component L homeolog Protein, NUF2, NDC80 kinetochore complex component S homeolog Protein, Nuf2 Protein, NUF2 Protein, nuf2 Protein, nuf2.L Protein, nuf2.S Protein
- Sujet
- Kinetochore protein Nuf2 (Cell division cycle-associated protein 1),FUNCTION: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. {ECO:0000250|UniProtKB:Q9BZD4}.
- Poids moléculaire
- 54.6 kDa
- UniProt
- Q99P69
- Pathways
- Maintenance of Protein Location
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