DPH2 Protein (AA 1-489) (Strep Tag)
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- Antigène Voir toutes DPH2 Protéines
- DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-489
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette DPH2 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MESTFSSPAE AALQREAGVP GQFTPPEDLD RVYELERVTK FVCDLGCQRV TLQFPDQLLG DAGAVAARLE EVTGAKMFIL GDTAYGSCCV DVLGAEQAGA QALVHFGPAC LSPPASQLPI TFVLGQRPVA LELCAKAFEA QNPDPTAPVV LLSEPACAHA LEPLAMLLLP KYQDLLISRP ALPLPVGSPS SQPESLERFG RCFPLNPGRR LEEYGAFYVG ASQASSDSSL DPDLSRLLLG WTPGRPFFSC CPDTGQTQDQ GAKAGRLRAR RLYLIERARD ARVVGLLAGT LGVARHREAL AHLRKLTEAA GKRSYVLAVG KPTPAKLANF PEMDVFVLLA CPLGALAPQP SGGFFRPVLT PCELEAACNP AWPPPGLAPH LTHYAELLPG SPFHVPLPPP ESELWDTPDV SLISGELRPP PPWKSSDDTR CSALIPRPQL ELAESSPAAS FLSSRNWQGL EPRLGQTPVK EAVRGRRGIA IAYEDEGSS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product DPH2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))
- Autre désignation
- Dph2 (DPH2 Produits)
- Synonymes
- DPH2L2 Protein, 9130020C19Rik Protein, AI467389 Protein, Dph2l2 Protein, id:ibd5058 Protein, zgc:162269 Protein, DPH2 homolog Protein, DPH2 homolog (S. cerevisiae) Protein, hypothetical protein Protein, DPH2 Protein, Dph2 Protein, dph2 Protein, CAALFM_C400690CA Protein
- Sujet
- 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 (Diphthamide biosynthesis protein 2) (Diphtheria toxin resistance protein 2) (MmDph2) (S-adenosyl-L-methionine:L-histidine 3-amino-3-carboxypropyltransferase DPH2),FUNCTION: Required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2 (PubMed:15485916). DPH1 and DPH2 transfer a 3-amino-3-carboxypropyl (ACP) group from S-adenosyl-L-methionine (SAM) to a histidine residue, the reaction is assisted by a reduction system comprising DPH3 and a NADH-dependent reductase (By similarity). Facilitates the reduction of the catalytic iron-sulfur cluster found in the DPH1 subunit (By similarity). {ECO:0000250|UniProtKB:P32461, ECO:0000269|PubMed:15485916}.
- Poids moléculaire
- 52.4 kDa
- UniProt
- Q9CR25
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