DPPA2 Protein (AA 1-301) (Strep Tag)
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- Antigène Voir toutes DPPA2 Protéines
- DPPA2 (Developmental Pluripotency Associated 2 (DPPA2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-301
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette DPPA2 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MSYFGLETFN ENQSEENLDE ESVILTLVPF KEEEEPNTDY ATQSNVSSST LDHTPPARSL VRHAGIKHPT RTIPSTCPPP SLPPIRDVSR NTLREWCRYH NLSTDGKKVE VYLRLRRHSY SKQECYIPNT SREARMKQGP KKSKIVFRGI GPPSGCQRKK EESGVLEILT SPKESTFAAW ARIAMRAAQS MSKNRCPLPS NVEAFLPQAT GSRWCVVHGR QLPADKKGWV RLQFLAGQTW VPDTPQRMNF LFLLPACIIP EPGVEDNLLC PECVHSNKKI LRNFKIRSRA KKNALPPNMP P
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product DPPA2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- DPPA2 (Developmental Pluripotency Associated 2 (DPPA2))
- Autre désignation
- Dppa2 (DPPA2 Produits)
- Synonymes
- 2410088E07Rik Protein, C80932 Protein, D19Mgi18 Protein, ECAT15-2 Protein, CT100 Protein, PESCRG1 Protein, developmental pluripotency associated 2 Protein, ABC-type oliopeptide/dipeptide transporter, periplasmic binding protein DppA Protein, Dppa2 Protein, DPPA2 Protein, dppA2 Protein
- Sujet
- Developmental pluripotency-associated protein 2 (Embryonic stem cell-associated transcript 15-2 protein) (ECAT15-2) (Pluripotent embryonic stem cell-related gene 1 protein),FUNCTION: Binds to target gene promoters, including NKX2-5 and SYCE1, but not GATA4, and may be involved in the maintenance of the active epigenetic status of these genes. {ECO:0000269|PubMed:21896782}.
- Poids moléculaire
- 34.1 kDa
- UniProt
- Q9CWH0
- Pathways
- Stem Cell Maintenance
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