HEYL Protein (AA 1-326) (Strep Tag)
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- Antigène Voir toutes HEYL Protéines
- HEYL (Hairy/enhancer-of-Split Related with YRPW Motif-Like (HEYL))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-326
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette HEYL protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MKRPRAPSGS DGESDGPIDV GQENDLSQMA RPLTTPSPSQ MQARKKRRGI IEKRRRDRIN SSLSELRRLV PTAFEKQGSS KLEKAEVLQM TVDHLKMLHA SGGTGFFDAR ALAVDFRSIG FRECLTEVIR YLGVLEGPSS HADPVRIRLL SHLKSYAAEM EPSPTTTSAL AFPVWPWSFL HSCPGLPSLN SQLAILGRVP GPVLPSISSP PYPISALRSA PVHRPAGTIP PTRRNLLPSR GVTSTQRAHL PERPAAPPPT ALDARAARSI VPIPPCSPTT APGAGKSDDN VSGSISSPCP SGPTGRPAGA VFYHSWVSEI TEIGAF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product HEYL Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- HEYL (Hairy/enhancer-of-Split Related with YRPW Motif-Like (HEYL))
- Autre désignation
- Heyl (HEYL Produits)
- Synonymes
- HESR3 Protein, HEY3 Protein, HRT3 Protein, bHLHb33 Protein, Hey3 Protein, Hrt3 Protein, hesr3 Protein, fk74e12 Protein, wu:fk74e12 Protein, hes related family bHLH transcription factor with YRPW motif-like Protein, hairy/enhancer-of-split related with YRPW motif-like Protein, hes-related family bHLH transcription factor with YRPW motif-like Protein, HEYL Protein, Heyl Protein, heyl Protein
- Sujet
- Hairy/enhancer-of-split related with YRPW motif-like protein (Hairy and enhancer of split-related protein 3) (Hairy-related transcription factor 3) (HRT-3) (mHRT3),FUNCTION: Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. {ECO:0000250, ECO:0000269|PubMed:16199874, ECO:0000269|PubMed:17303760}.
- Poids moléculaire
- 34.9 kDa
- UniProt
- Q9DBX7
- Pathways
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling
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