Gasdermin A Protein (GSDMA) (AA 1-446) (Strep Tag)
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- Antigène Voir toutes Gasdermin A (GSDMA) Protéines
- Gasdermin A (GSDMA)
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-446
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette Gasdermin A protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Séquence
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MTMFENVTRA LARQLNPRGD LTPLDSLIDF KRFHPFCLVL RKRKSTLFWG ARYVHTDYTL LDVLEPGSSP SDPTDSGNFS FKNMLDARVE GDVDVPKTVK VKGTAGLSRS STLEVQTLSV APTALENLHK ERKLSADHPF LKEMRERGEN LYVVMEVVET LQEVTLERAG KAEGCFSLPF FAPLGLQGSV NHKEAVTIPK GCVLAYRVRQ LMVNGKDEWG IPHICNDSMQ TFPPGEKPGE GKFILIQASD VGEMHEDFKT LKEEVQRETQ EVEKLSPVGR SSLLTSLSHL LGKKKELQDL EQTLEGALDK GHEVTLEALP KDVLLSKDAM DAILYFLGAL TVLSEAQQKL LVKSLEKKIL PVQLKLVEST MEKNFLQDKE GVFPLQPDLL SSLGEEELIL TEALVGLSGL EVQRSGPQYT WDPDTLPHLC ALYAGLSLLQ LLSKNS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product GSDMA Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Gasdermin A (GSDMA)
- Autre désignation
- Gsdma (GSDMA Produits)
- Synonymes
- BB149167 Protein, Gsdm Protein, Gsdm1 Protein, Gsdma1 Protein, H312E Protein, GSDM1 Protein, GSDM Protein, gasdermin A Protein, Gsdma Protein, GSDMA Protein
- Sujet
- Gasdermin-A (Gasdermin-1) (Gasdermin-A1) [Cleaved into: Gasdermin-A, N-terminal (GSDMA-NT), Gasdermin-A, C-terminal (GSDMA-CT)],FUNCTION: [Gasdermin-A]: This form constitutes the precursor of the pore-forming protein and acts as a sensor of bacterial infection: upon infection, specifically cleaved by bacterial effector protein SpeB in epithelial cells, releasing the N-terminal moiety (Gasdermin-A, N-terminal) that binds to membranes and forms pores, triggering pyroptosis. {ECO:0000269|PubMed:35110732}., FUNCTION: [Gasdermin-A, N-terminal]: Pore-forming protein that causes membrane permeabilization and pyroptosis (PubMed:35110732). Released upon cleavage by bacterial effector protein SpeB, and binds to membrane inner leaflet lipids (PubMed:35110732). Homooligomerizes within the membrane and forms pores of 10-15 nanometers (nm) of inner diameter, triggering pyroptosis (By similarity). Pyroptosis triggers the elimination of the infected skin cell, depriving the pathogen of its protective niche, while inducing an inflammatory response (PubMed:35110732, PubMed:35545676). This ultimately prevents bacterial penetration of the epithelial barrier and a subsequent systemic dissemination of the pathogen (PubMed:35110732, PubMed:35545676). Binds to cardiolipin and other acidic phospholipids, such as phosphatidylserine, which mediate its targeting to the inner leaflet membrane (By similarity). {ECO:0000250|UniProtKB:Q96QA5, ECO:0000269|PubMed:35110732, ECO:0000269|PubMed:35545676}.
- Poids moléculaire
- 49.6 kDa
- UniProt
- Q9EST1
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