ADRM1 Protein (AA 2-407) (His tag)
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- Antigène Voir toutes ADRM1 (Adrm1) Protéines
- ADRM1 (Adrm1) (Adhesion Regulating Molecule 1 (Adrm1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-407
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Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette ADRM1 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- Séquence
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TTSGALFPSL VPGSRGSSTK YLVEFRAGKM SLKGTTVTPD KRKGLVYIQQ TDDSLIHFCW KDRTSGTVED DLIIFPDDCE FKRVPQCPSG RVYVLKFKAG SKRLFFWMQE PKTDQDEEHC RKVNECLNNP PMPGSLGASG SSGHELSALG GEGGLQSLLG NMSHSQLMQL IGPAGLGGLG GLGALTGPGL ASLLGSSGPP ASSSSSSSRS QSAAVTPSSS TSSARATPAP SAPAAASATS PSPAPSSGNG TSTAASPTQP IQLSDLQSIL ATMNVPAGPG GSQQVDLASV LTPEIMAPIL ANADVQERLL PYLPSGESLP QTADEIQNTL TSPQFQQALG MFSAALASGQ LGPLMCQFGL PAEAVEAANK GDVEAFAKAM QNNAKSDPKE GDTKDKKDEE EDMSLD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Adrm1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product Adrm1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ADRM1 (Adrm1) (Adhesion Regulating Molecule 1 (Adrm1))
- Autre désignation
- Adrm1 (Adrm1 Produits)
- Synonymes
- arm-1 Protein, gp110 Protein, rpn13 Protein, xoom Protein, ADRM1 Protein, adrm1 Protein, ARM-1 Protein, ARM1 Protein, GP110 Protein, 1110063P18Rik Protein, 2510006J17Rik Protein, AA408205 Protein, AU043535 Protein, Arm1 Protein, Gp110 Protein, Rpn13 Protein, adrm1a Protein, adrm1b Protein, fa12d09 Protein, wu:fa10g10 Protein, wu:fa12d09 Protein, zgc:56107 Protein, zgc:77256 Protein, adhesion regulating molecule 1 Protein, ADRM1 Protein, 19S proteasome regulatory subunit Protein, ADRM1 protein Protein, adrm1 Protein, ADRM1 Protein, CpipJ_CPIJ002929 Protein, SJAG_04211 Protein, Adrm1 Protein
- Sujet
- Functions as a proteasomal ubiquitin receptor. Recruits the deubiquitinating enzyme UCHL5 at the 26S proteasome and promotes its activity. {ECO:0000269|PubMed:15819879, ECO:0000269|PubMed:18497827}.
- Poids moléculaire
- 42.9 kDa Including tag.
- UniProt
- Q9JKV1
- Pathways
- Positive Regulation of Endopeptidase Activity
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