TRAPPC11 Protein (AA 1-1133) (Strep Tag)
-
- Antigène Voir toutes TRAPPC11 Protéines
- TRAPPC11 (Trafficking Protein Particle Complex 11 (TRAPPC11))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1133
- Origine
- Souris
-
Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette TRAPPC11 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
-
MSPTQWDFPV ELCCRPMAFV TLTGLDVVYN AVHRAVWDAF CANRRADRVP ISFKVLPGDH EYPKCRSKRT SYEWYIPKGV LKTGWMNKHL NLVPALVVVF YELDWDEPQW KEKQSECATR VEIVRQSLQG RNTKVAVVLI QKKTPLPPGE DVIASERAAA LCNVCELSGK SLFVLPHTDH LVGYIIRLEN AFYEHAQTYY YTEIRRVKSH KEFLNKTTHQ LLFVRHQFKI AFFSELKQDT QNALKNYRIA YNLVHELRAH ETNILEIKTM AGFINYKICR LCFQHNTPLD AIAQFRKHID LCKKKIGSAE LAFEHAAWMA KQFQAFGDLF DEAIKLGLTA IQTQNPGFYY QQAAYYAQER KQHAKALCNH DAAVMYPNPD PLETQSGVLD FYGQRPWRQG ILSFDLSDPE KEKAGILAIQ LKERSVVHSE IIIALLSNAV AQFKKYKCPR MKSHLMVQMG EEYYYAKDYT KALKLLDYVM CDYRSEAWWT LLTSILTTAL KCSYLMAQLK DYITYSLELL GRASTLKDEQ KSRIEKNLMN VLMNESPDPE PDCDVLAVKT AQKLWADRVS LAGSNVFQIG VQDFVPFVQC KAKFHAPSFH VDVPVEFDVF LKADCPHPIR FSKLCVSFNN QVYNQFCVLE EASKASEVLE NLTQGKMCLV PGKTRKLSFK FVAKTEDVGK KIEITSVDLF LGSESGRCVV LSWQGGGGDA ASSQEALQAA RSFKRRPKLA EDEIHWDSVI IQASTMIISR VPNISVHLRH EPPALMNEMY CLVVTVQSHE KSPIRDVKLT AGLKPGQDAN LTQKTHVTLH GAELCDESYP ALLTDIPVGD LHPGEQLEKT VYVRCGTVGS RMFLVYVSYL INTTVEGKEI ICKCHKDDTV TIETVFPFDV AVRFVSTKFE HLERVYADIP FLLMTDVLSA SPWALTIVSS ELQLAPSMTA MDHLESQIDK VVLQTGESAS ECFCLRCPSA GNIEGGVATG HYIISWKRAS VVESIPAVST VITLPHVIAE NIPLHVNADL PSFGRVRESL PVRYHLQNKT DLVQDVEISV EPSDAFMFSG LKQIRLRILP GTKQEMLYNF YPLMAGYQQL PSLNINLLRF PNFTNQLLRR FIPTSIFVKP QGRLLEDTSI AAA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product TRAPPC11 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- TRAPPC11 (Trafficking Protein Particle Complex 11 (TRAPPC11))
- Autre désignation
- Trappc11 (TRAPPC11 Produits)
- Synonymes
- TRAPPC11 Protein, C4orf41 Protein, FOIGR Protein, GRY Protein, LGMD2S Protein, C27H4orf41 Protein, AW558465 Protein, D030016E14Rik Protein, R75422 Protein, fb78g05 Protein, fgr Protein, flj12716l Protein, foigr Protein, wu:fb78g05 Protein, zgc:136723 Protein, zgc:63684 Protein, trafficking protein particle complex 11 Protein, TRAPPC11 Protein, Trappc11 Protein, trappc11 Protein
- Sujet
- Trafficking protein particle complex subunit 11,FUNCTION: Involved in endoplasmic reticulum to Golgi apparatus trafficking at a very early stage. {ECO:0000250}.
- Poids moléculaire
- 128.4 kDa
- UniProt
- B2RXC1
-