EXOC7 Protein (AA 1-697) (Strep Tag)
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- Antigène Voir toutes EXOC7 Protéines
- EXOC7 (Exocyst Complex Component 7 (EXOC7))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-697
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette EXOC7 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MIPPQEASAR RREIEDKLKQ EEETLSFIRD SLEKSDQLTR NMVSILSSFE SRLMKLENSI IPVHKQTENL QRLQENVEKT LSCLDHVISY YHVASDTEKI IREGPTGRLE EYLGSMAKIQ KAVEYFQDNS PDSPELNKVK LLFERGKESL ESEFRSLMTR HSKVVSPVLL LDLISADDEL EVQEDVVLEH LPESVLRDVV RISRWLVEYG RNQDFMNVYY QIRSSQLDRS IKGLKEHFRK SSSSSGVPYS PAIPNKRKDT PTKKPIKRPG TIRKAQNLLK QYSQHGLDGK KGGSNLIPLE GRDDMLDVET DAYIHCVSAF VKLAQSEYRL LMEIIPEHHQ KKTFDSLIQD ALDGLMLEGE NIVSAARKAI IRHDFSTVLT VFPILRHLKQ TKPEFDQVLQ GTAASTKNKL PGLITSMETI GAKALEDFAD NIKNDPDKEY NMPKDGTVHE LTSNAILFLQ QLLDFQETAG AMLASQVLGD TYNIPLDPRE TSSSATSYSS EFSKRLLSTY ICKVLGNLQL NLLSKSKVYE DPALSAIFLH NNYNYILKSL EKSELIQLVA VTQKTAERSY REHIEQQIQT YQRSWLKVTD YIAEKNLPVF QPGVKLRDKE RQMIKERFKG FNDGLEELCK IQKVWAIPDT EQRDKIRQAQ KDIVKETYGA FLHRYGSVPF TKNPEKYIKY RVEQVGDMID RLFDTSA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product EXOC7 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- EXOC7 (Exocyst Complex Component 7 (EXOC7))
- Autre désignation
- Exoc7 (EXOC7 Produits)
- Synonymes
- 2-5-3p Protein, EX070 Protein, EXO70 Protein, EXOC1 Protein, Exo70p Protein, YJL085W Protein, Exo70 Protein, sec70 Protein, zgc:56575 Protein, exoc7 Protein, EXOC7 Protein, DDBDRAFT_0217074 Protein, DDBDRAFT_0233927 Protein, DDB_0217074 Protein, DDB_0233927 Protein, DKFZp459C0924 Protein, exocyst complex component 7 Protein, exocyst complex subunit 7 Protein, exocyst complex component 7 L homeolog Protein, EXOC7 Protein, Exoc7 Protein, exoc7 Protein, exoc7.L Protein
- Sujet
- Exocyst complex component 7 (Exocyst complex component Exo70),FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion. It is required for neuron survival and plays an essential role in cortical development (By similarity). {ECO:0000250|UniProtKB:E7FC72, ECO:0000269|PubMed:12687004}.
- Poids moléculaire
- 80.0 kDa
- UniProt
- O35250
- Pathways
- Peptide Hormone Metabolism, ER-Nucleus Signaling, Synaptic Vesicle Exocytosis
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