Sorting Nexin 3 Protein (SNX3) (AA 2-162) (His tag)
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- Antigène Voir toutes Sorting Nexin 3 (SNX3) Protéines
- Sorting Nexin 3 (SNX3)
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-162
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Origine
- Souris
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Source
- Escherichia coli (E. coli)
- Purification/Conjugué
- Cette Sorting Nexin 3 protéine est marqué à la His tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
- Séquence
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AETVADTRRL ITKPQNLNDA YGPPSNFLEI DVSNPQTVGV GRGRFTTYEI RVKTNLPIFK LKESTVRRRY SDFEWLRSEL ERESKVVVPP LPGKAFLRHV PFRGDDGIFD DNFIEERKQG LEQFINKVAG HPLAQNERCL HMFLQDEIID KSYTPSKIRH A
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Snx3 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product SNX3 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Sorting Nexin 3 (SNX3)
- Autre désignation
- Snx3 (SNX3 Produits)
- Synonymes
- SNX3 Protein, sdp3 Protein, Grd19 Protein, snx3a Protein, wu:fb53a08 Protein, zgc:123189 Protein, MCOPS8 Protein, SDP3 Protein, sorting nexin 3 Protein, sorting nexin 3 L homeolog Protein, potential phosphoinositide-binding protein similar to S. cerevisiae GRD19 (YOR357C) late Golgi protein involved in retrieval of proteins from prevacuolar compartment; same as cosmid orf Ca41C10.03 Protein, SNX3 Protein, snx3.L Protein, snx3 Protein, GRD19 Protein, Snx3 Protein
- Sujet
- Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Also can bind phosphatidylinositol 4-phosphate (PtdIns(P4)), phosphatidylinositol 5-phosphate (PtdIns(P5)) and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2) (PubMed:19576982). Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC). May act in part as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway (By similarity). Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (PubMed:18632802). Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes (By similarity). Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor Tfrc presuambly by delivering the tranferrin:transferrin receptor complex to recycling endosomes, the function may involve the CSC retromer subcomplex (PubMed:23416069). Involved in regulation of neurite outgrowth in primary neurons (PubMed:19576982). {ECO:0000250|UniProtKB:O60493, ECO:0000269|PubMed:18632802, ECO:0000269|PubMed:23416069}.
- Poids moléculaire
- 19.6 kDa Including tag.
- UniProt
- O70492
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