PAPSS2 Protein (AA 1-621) (Strep Tag)
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- Antigène Voir toutes PAPSS2 Protéines
- PAPSS2 (3'-phosphoadenosine 5'-phosphosulfate Synthase 2 (PAPSS2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-621
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette PAPSS2 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MSANFKMNHK RDQQKSTNVV YQAHHVSRNK RGQVVGTRGG FRGCTVWLTG LSGAGKTTIS FALEEYLVSH AIPCYSLDGD NVRHGLNKNL GFSAGDREEN IRRIAEVARL FADAGLVCIT SFISPFAKDR ENARKIHESA GLPFFEIFVD APLNICESRD VKGLYKRARA GEIKGFTGID SDYEKPETPE CVLKTNLSSV SDCVQQVVEL LQEQNIVPHT TIKGIHELFV PENKVDQIRA EAETLPSLPI TKLDLQWVQI LSEGWATPLK GFMREKEYLQ TLHFDTLLDG VVPRDGVINM SIPIVLPVSA DDKARLEGCS KFALMYEGRR VALLQDPEFY EHRKEERCSR VWGTATAKHP HIKMVMESGD WLVGGDLQVL ERIRWDDGLD QYRLTPLELK QKCKDMNADA VFAFQLRNPV HNGHALLMQD TRRRLLERGY KHPVLLLHPL GGWTKDDDVP LEWRMKQHAA VLEERVLDPK STIVAIFPSP MLYAGPTEVQ WHCRCRMIAG ANFYIVGRDP AGMPHPETKK DLYEPTHGGK VLSMAPGLTS VEIIPFRVAA YNKIKKAMDF YDPARHEEFD FISGTRMRKL AREGEDPPDG FMAPKAWKVL TDYYRSLEKT N
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product PAPSS2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- PAPSS2 (3'-phosphoadenosine 5'-phosphosulfate Synthase 2 (PAPSS2))
- Autre désignation
- Papss2 (PAPSS2 Produits)
- Synonymes
- ATPSK2 Protein, BCYM4 Protein, SK2 Protein, 1810018P12Rik Protein, AI159688 Protein, AtpsU2 Protein, Atpsk2 Protein, Sk2 Protein, bm Protein, Papss1 Protein, id:ibd2761 Protein, papss2 Protein, sb:cb868 Protein, wu:fb12e05 Protein, zgc:55851 Protein, zgc:85655 Protein, PAPSS2 Protein, zgc:153748 Protein, 3'-phosphoadenosine 5'-phosphosulfate synthase 2 Protein, 3'-phosphoadenosine 5'-phosphosulfate synthase 2b Protein, 3'-phosphoadenosine 5'-phosphosulfate synthase 2a Protein, PAPSS2 Protein, Papss2 Protein, papss2b Protein, papss2.S Protein, LOAG_05620 Protein, papss2 Protein, papss2a Protein
- Sujet
- Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 (PAPS synthase 2) (PAPSS 2) (Sulfurylase kinase 2) (SK 2) (SK2) [Includes: Sulfate adenylyltransferase (EC 2.7.7.4) (ATP-sulfurylase) (Sulfate adenylate transferase) (SAT), Adenylyl-sulfate kinase (EC 2.7.1.25) (3'-phosphoadenosine-5'-phosphosulfate synthase) (APS kinase) (Adenosine-5'-phosphosulfate 3'-phosphotransferase) (Adenylylsulfate 3'-phosphotransferase)],FUNCTION: Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate/PAPS, the activated sulfate donor used by sulfotransferases (PubMed:10559207). In mammals, PAPS is the sole source of sulfate while APS appears to only be an intermediate in the sulfate-activation pathway. May have an important role in skeletogenesis during postnatal growth. {ECO:0000269|PubMed:10559207}.
- Poids moléculaire
- 70.4 kDa
- UniProt
- O88428
- Pathways
- Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process
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