ORC4 Protein (AA 1-433) (Strep Tag)
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- Antigène Voir toutes ORC4 Protéines
- ORC4 (Origin Recognition Complex, Subunit 4 (ORC4))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-433
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ORC4 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MSSRKTKSNA HAECLSQVQR ILRERFCHHS PHSNLFGVQV QYKHLIELLK RTAIYGESNS VLIVGPRGSG KTTLLNHALK ELMEIEVSEN VIQVHLNGLL QTNEKIALKE ITRQLNLDNV VEDKVFGSFA ENLSFLLEAL QKGDRTSSCP VIFILDEFDI FAHQKNQTLL YNLFDISQSA QTPVAVIGLT CRLDILELLE KRVKSRFSHR QIHLMNSFDF PQYLKIFKEQ LSLPAEFPDK AFAERWNENV HCLSEDSTVL EVLQKHFSVN KNLQSLHMLL MLALNRVTVS HPFMTSADLM EAQHMCSLDS KANIVHGLSV LEICLIIAMK HLNDIYEEEP FNFQMVYNEF QKFIQRKAHS VYNFEKPVVM KAFEHLQQLE LIKPVERTSV NSQREYQLVK LLLDNTQIMN ALQKYSNCPT DVRQWATSSL SWL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product ORC4 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ORC4 (Origin Recognition Complex, Subunit 4 (ORC4))
- Autre désignation
- Orc4 (ORC4 Produits)
- Synonymes
- ORC4L Protein, ORC4P Protein, Orc4P Protein, Orc4l Protein, mMmORC4 Protein, CG2917 Protein, DmORC4 Protein, Dmel\\CG2917 Protein, ORC Protein, ORC4 Protein, dmOrc4 Protein, orc4 Protein, rDmORC Protein, orc4l Protein, fc50c03 Protein, wu:fc50c03 Protein, zgc:85772 Protein, ATORC4 Protein, F23H14.9 Protein, F23H14_9 Protein, ORIGIN RECOGNITION COMPLEX SUBUNIT 4 Protein, origin recognition complex subunit 4 Protein, origin recognition complex subunit 4 Protein, origin recognition complex, subunit 4 Protein, Origin recognition complex subunit 4 Protein, origin recognition complex subunit 4 L homeolog Protein, origin recognition complex subunit Orc4 Protein, ORC4 Protein, Orc4 Protein, orc4.L Protein, orc4 Protein, CNM01830 Protein
- Sujet
- Origin recognition complex subunit 4,FUNCTION: Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity). Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. {ECO:0000250}.
- Poids moléculaire
- 50.0 kDa
- UniProt
- O88708
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA
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