NFATC1 Protein (AA 1-717) (Strep Tag)
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- Antigène Voir toutes NFATC1 Protéines
- NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-717
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette NFATC1 protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Séquence
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MPNTSFPVPS KFPLGPPAAV CGSGETLRPA PPSGGTMKAA EEEHYSYVSP SVTSTLPLPT AHSALPAACH DLQTSTPGIS AVPSANHPPS YGGAVDSGPS GYFLSSGNTR PNGAPTLESP RIEITSYLGL HHGSGQFFHD VEVEDVLPSC KRSPSTATLH LPSLEAYRDP SCLSPASSLS SRSCNSEASS YESNYSYPYA SPQTSPWQSP CVSPKTTDPE EGFPRSLGAC HLLGSPRHSP STSPRASITE ESWLGARGSR PTSPCNKRKY SLNGRQPSCS PHHSPTPSPH GSPRVSVTED TWLGNTTQYT SSAIVAAINA LTTDSTLDLG DGVPIKSRKT ALEHAPSVAL KVEPAGEDLG TTPPTSDFPP EEYTFQHLRK GAFCEQYLSV PQASYQWAKP KSLSPTSYMS PSLPALDWQL PSHSGPYELR IEVQPKSHHR AHYETEGSRG AVKASAGGHP IVQLHGYLEN EPLTLQLFIG TADDRLLRPH AFYQVHRITG KTVSTTSHEI ILSNTKVLEI PLLPENNMRA IIDCAGILKL RNSDIELRKG ETDIGRKNTR VRLVFRVHIP QPNGRTLSLQ VASNPIECSQ RSAQELPLVE KQSTDSYPVI GGKKMVLSGH NFLQDSKVIF VEKAPDGHHV WEMEAKTDRD LCKPNSLVVE IPPFRNQRIT SPAQVSFYVC NGKRKRSQYQ RFTYLPANGN SVFLTLSSES ELRGGFY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product NFATC1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))
- Autre désignation
- Nfatc1 (NFATC1 Produits)
- Synonymes
- nf-atc Protein, nfat2 Protein, nfatc Protein, si:ch211-198c22.2 Protein, NFATC1 Protein, 2210017P03Rik Protein, AI449492 Protein, AV076380 Protein, NF-ATc Protein, NFAT2 Protein, NFATc Protein, Nfatcb Protein, NF-ATC Protein, NF-ATc1 Protein, NFAT Protein, NFATmac Protein, nuclear factor of activated T-cells 1 Protein, nuclear factor of activated T-cells 1 L homeolog Protein, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 Protein, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1 Protein, NFATC1 Protein, nfatc1.L Protein, nfatc1 Protein, Nfatc1 Protein
- Sujet
- Nuclear factor of activated T-cells, cytoplasmic 1 (NF-ATc1) (NFATc1) (NFAT transcription complex cytosolic component) (NF-ATc) (NFATc),FUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription (PubMed:9388475). Also controls gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of T-lymphocytes as well as lymphoid and non-lymphoid cells (By similarity). Required for osteoclastogenesis and regulates many genes important for osteoclast differentiation and function (PubMed:23990468, PubMed:26644563). {ECO:0000250|UniProtKB:O95644, ECO:0000269|PubMed:23990468, ECO:0000269|PubMed:9388475, ECO:0000303|PubMed:26644563}.
- Poids moléculaire
- 77.8 kDa
- UniProt
- O88942
- Pathways
- Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway
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