MPRIP Protein (AA 1-1024) (Strep Tag)
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- Antigène Tous les produits MPRIP
- MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1024
- Origine
- Souris
- Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette MPRIP protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL LLAPDGTDFD NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM NQCTDVVDGE ARTGQKFSLC ILTPDKEHFI RAETKEIISG WLEMLMVYPR TNKQNQKKKR KVEPPTPQEP GPAKMAVTSS SGGSSGSSSS IPSAEKVPTT KSTLWQEEMR AKDQPDGTSL SPAQSPSQSQ PPAACTPREP GLESKEDEST ISGDRVDGGR KVRVESGYFS LEKAKQDLRA EEQLPPLLSP PSPSTPHSRR SQVIEKFEAL DIEKAEHMET NMLILTTPSS DTRQGRSERR AIPRKRDFAS EAPTAPLSDA CPLSPHRRAK SLDRRSTESS MTPDLLNFKK GWLTKQYEDG QWKKHWFVLA DQSLRYYRDS VAEEAADLDG EINLSTCYDV TEYPVQRNYG FQIHTKEGEF TLSAMTSGIR RNWIQTIMKH VLPASAPDVT SSLPEGKNKS TSFETCSRST EKQEAEPGEP DPEQKKSRAR ERRREGRSKT FDWAEFRPIQ QALAQERASA VGSSDSGDPG CLEAEPGELE RERARRREER RKRFGMLDTI DGPGMEDTAL RMDIDRSPGL LGTPDLKTQN VHVEIEQRWH QVETTPLREE KQVPIAPLHL SLEDRSERLS THELTSLLEK ELEQSQKEAS DLLEQNRLLQ DQLRVALGRE QSAREGYVLQ ATCERGFAAM EETHQKKIED LQRQHQRELE KLREEKDRLL AEETAATISA IEAMKNAHRE EMERELEKSQ RSQISSINSD IEALRRQYLE ELQSVQRELE VLSEQYSQKC LENAHLAQAL EAERQALRQC QRENQELNAH NQELNNRLAA EITRLRTLLT GDGGGESTGL PLTQGKDAYE LEVLLRVKES EIQYLKQEIS SLKDELQTAL RDKKYASDKY KDIYTELSIA KAKADCDISR LKEQLKAATE ALGEKSPEGT TVSGYDIMKS KSNPDFLKKD RSCVTRQLRN IRSKSVIEQV SWDN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- MPRIP (Myosin Phosphatase rho Interacting Protein (MPRIP))
- Autre désignation
- Mprip (MPRIP Produits)
- Synonymes
- M-RIP Protein, MRIP Protein, RHOIP3 Protein, RIP3 Protein, p116Rip Protein, 9530046C02 Protein, AA536749 Protein, AI647711 Protein, C76423 Protein, Rhoip3 Protein, mKIAA0864 Protein, M-rip Protein, Mrip Protein, myosin phosphatase Rho interacting protein Protein, MPRIP Protein, Mprip Protein
- Sujet
- Myosin phosphatase Rho-interacting protein (Rho-interacting protein 3) (RIP3) (p116Rip),FUNCTION: Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells. {ECO:0000269|PubMed:12732640, ECO:0000269|PubMed:15469989}.
- Poids moléculaire
- 116.4 kDa
- UniProt
- P97434
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