GRIN2B Protein (AA 839-1482) (His tag)
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- Antigène Voir toutes GRIN2B Protéines
- GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 839-1482
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Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette GRIN2B protéine est marqué à la His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
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EHLFYWQFRH CFMGVCSGKP GMVFSISRGI YSCIHGVAIE ERQSVMNSPT ATMNNTHSNI LRLLRTAKNM ANLSGVNGSP QSALDFIRRE SSVYDISEHR RSFTHSDCKS YNNPPCEENL FSDYISEVER TFGNLQLKDS NVYQDHYHHH HRPHSIGSTS SIDGLYDCDN PPFTTQPRSI SKKPLDIGLP SSKHSQLSDL YGKFSFKSDR YSGHDDLIRS DVSDISTHTV TYGNIEGNAA KRRKQQYKDS LKKRPASAKS RREFDEIELA YRRRPPRSPD HKRYFRDKEG LRDFYLDQFR TKENSPHWEH VDLTDIYKER SDDFKRDSVS GGGPCTNRSH LKHGTGDKHG VVGGVPAPWE KNLTNVDWED RSGGNFCRSC PSKLHNYSST VAGQNSGRQA CIRCEACKKA GNLYDISEDN SLQELDQPAA PVAVSSNAST TKYPQSPTNS KAQKKNRNKL RRQHSYDTFV DLQKEEAALA PRSVSLKDKG RFMDGSPYAH MFEMPAGESS FANKSSVTTA GHHHNNPGSG YMLSKSLYPD RVTQNPFIPT FGDDQCLLHG SKSYFFRQPT VAGASKTRPD FRALVTNKPV VSALHGAVPG RFQKDICIGN QSNPCVPNNK NPRAFNGSSN GHVYEKLSSI ESDV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Grin2b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product GRIN2B Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
- Autre désignation
- Grin2b (GRIN2B Produits)
- Synonymes
- NMDAR2B Protein, GluN2B Protein, MRD6 Protein, NR2B Protein, hNR3 Protein, nmdar2b Protein, nr2b Protein, AW490526 Protein, Nmdar2b Protein, glutamate ionotropic receptor NMDA type subunit 2B Protein, glutamate receptor, ionotropic, N-methyl D-aspartate 2B L homeolog Protein, glutamate receptor, ionotropic, NMDA2B (epsilon 2) Protein, GRIN2B Protein, Grin2b Protein, grin2b.L Protein
- Sujet
- NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death. {ECO:0000269|PubMed:20141836}.
- Poids moléculaire
- 73.3 kDa Including tag.
- UniProt
- Q01097
- Pathways
- Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
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