AGBL5 Protein (AA 1-886) (Strep Tag)
-
- Antigène Voir toutes AGBL5 Protéines
- AGBL5 (ATP/GTP Binding Protein-Like 5 (AGBL5))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-886
- Origine
- Souris
-
Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette AGBL5 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
-
MELRCGGLLF SSRFDSGNLA HVEKVETVSS DGEGVGGVAT APASGSAASP DYEFNVWTRP DCAETEYENG NRSWFYFSVR GGTPGKLIKI NIMNMNKQSK LYSQGMAPFV RTLPSRPRWE RIRERPTFEM TETQFVLSFV HRFVEGRGAT TFFAFCYPFS YSDCQDLLSQ LDQRFSENYS THSSPLDSIY YHRELLCYSL DGLRVDLLTI TSCHGLRDDR EPRLEQLFPD LGTPRPFRFT GKRIFFLSSR VHPGETPSSF VFNGFLDFIL RPDDPRAQTL RRLFVFKLIP MLNPDGVVRG HYRTDSRGVN LNRQYLKPDA VLHPAIYGAK AVLLYHHVHS RLNAKSPTNQ QPTLHLPPEA PLSDLEKANN LHNEAHLGQS PDGENPATWP ETEPAEEKTD PVWLMPQPIP ELEEPAPDTI PPKESGVAYY VDLHGHASKR GCFMYGNSFS DESTQVENML YPKLISLNSA HFDFQGCNFS EKNMYARDRR DGQSKEGSGR VAIYKASGII HSYTLECNYN TGRSVNSIPA ACHDNGRASP PPPPAFPSRY TVELFEQVGR AMAIAALDMA ECNPWPRIVL SEHSSLTNLR AWMLRHVRNS RGLTSAGNMG ASKKRGARTP PKSNNSLPVS CSENALSRVR SFSTGTSTGG SSSSQQNSPQ MKNSPSFPFH GSRTAGLPGL GSSTQKVSHR VLGPVREPRC SDRRRRQQPL NHRSTTSSLA PSPTLASSGP TSSRNMGSCL LPNSLSLSGS SCSFSSSGDK PEAVMVIGKS LLGAGARIPC IRTRLQARPR LGRSSPPTRR GMRGSSPTSP IPQTRESSEL EPGPHSATPG LPQAGPPRPR SAPAFSPISC TLSDSPSRIC YSRGLLNQCE VCFVPKSPPL TISPRV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product AGBL5 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- AGBL5 (ATP/GTP Binding Protein-Like 5 (AGBL5))
- Autre désignation
- Agbl5 (AGBL5 Produits)
- Synonymes
- zgc:91997 Protein, MGC83526 Protein, AGBL5 Protein, ccp5 Protein, 4930455N08 Protein, 9430057O19Rik Protein, CCP5 Protein, ATP/GTP binding protein-like 5 Protein, ATP/GTP binding protein-like 5 L homeolog Protein, ATP/GTP binding protein like 5 Protein, cytosolic carboxypeptidase-like protein 5 Protein, agbl5 Protein, agbl5.L Protein, AGBL5 Protein, LOC100181588 Protein, Agbl5 Protein
- Sujet
- Cytosolic carboxypeptidase-like protein 5 (EC 3.4.17.-) (EC 3.4.17.24) (ATP/GTP-binding protein-like 5) (Protein deglutamylase CCP5),FUNCTION: Metallocarboxypeptidase that mediates deglutamylation of tubulin and non-tubulin target proteins (PubMed:21074048, PubMed:20519502, PubMed:24022482, PubMed:26829768). Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin (PubMed:21074048, PubMed:20519502, PubMed:24022482). Cleaves alpha- and gamma-linked polyglutamate tubulin side-chain, as well as the branching point glutamate (PubMed:21074048, PubMed:24022482). Also catalyzes the removal of alpha-linked glutamate residues from the carboxy-terminus of alpha-tubulin (PubMed:24022482). Mediates deglutamylation of nucleotidyltransferase CGAS, leading to CGAS antiviral defense response activation (PubMed:26829768). {ECO:0000269|PubMed:20519502, ECO:0000269|PubMed:21074048, ECO:0000269|PubMed:24022482, ECO:0000269|PubMed:26829768}.
- Poids moléculaire
- 97.6 kDa
- UniProt
- Q09M02
-