PRDM6 Protein (AA 1-596) (Strep Tag)
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- Antigène Voir toutes PRDM6 Protéines
- PRDM6 (PR Domain Containing 6 (PRDM6))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-596
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette PRDM6 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MLKPGDPGGS AFLKVDPAYL QHWQQLFPHG GGGGGPLKAS GAALALGAPQ PLQPPPPPPP PPPERAEPPP DGLRPRPASL SSTPAPSSTS ASSASSCAAA AAAAALAGLS ALPVAQMPVF APLAAAAVAA EPLPPKDLCL GASAGPGPAK CGGGGGSVGD GRGVPRFRCS AEELDYYLYG QQRMEIIPLN QHTSDPNNRC DMCADNRNGE CPMHGPLHSL RRLVGTSSAA AAAPPPELPE WLRDLPREVC LCTSTVPGLA YGICAAQRIQ QGTWIGPFQG VLLSPEKVQT GVVRNTQHLW EIYDQDGTLQ HFIDGGEPSK SSWMRYIRCA RHCGEQNLTV VQYRSNIFYR ACIDIPRGTE LLVWYNDSYT SFFGIPLQCI AQDENLNVPS TVMEAMCRQD ALQPFNKSSK LSPSGQQRSV VFPQTPCSRN FSLLDKSGPM EAGFNQINVK NQRVLASPTS TSQLHSEFSD WHLWKCGQCF KTFTQRILLQ MHVCTQNPDR PYQCGHCSQS FSQPSELRNH VVTHSSDRPF KCGYCGRAFA GATTLNNHIR THTGEKPFKC ERCERSFTQA TQLSRHQRMP NECKPITESP ESIEVD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product PRDM6 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- PRDM6 (PR Domain Containing 6 (PRDM6))
- Autre désignation
- Prdm6 (PRDM6 Produits)
- Synonymes
- Gm92 Protein, PRISM Protein, RGD1559594 Protein, PRDM6 Protein, PR/SET domain 6 Protein, PR domain containing 6 Protein, putative histone-lysine N-methyltransferase PRDM6 Protein, PRDM6 Protein, Prdm6 Protein, LOC100354345 Protein, prdm6 Protein
- Sujet
- Putative histone-lysine N-methyltransferase PRDM6 (EC 2.1.1.361) (PR domain zinc finger protein 6) (PR domain-containing protein 6) (PR domain-containing protein in smooth muscle),FUNCTION: Putative histone methyltransferase that acts as a transcriptional repressor of smooth muscle gene expression (PubMed:16537907, PubMed:17662997). Promotes the transition from differentiated to proliferative smooth muscle by suppressing differentiation and maintaining the proliferative potential of vascular smooth muscle cells (PubMed:27181681, PubMed:16537907, PubMed:17662997). Also plays a role in endothelial cells by inhibiting endothelial cell proliferation, survival and differentiation. It is unclear whether it has histone methyltransferase activity in vivo. According to some authors, it does not act as a histone methyltransferase by itself and represses transcription by recruiting EHMT2/G9a (PubMed:16537907). According to others, it possesses histone methyltransferase activity when associated with other proteins and specifically methylates 'Lys-20' of histone H4 in vitro. 'Lys-20' methylation represents a specific tag for epigenetic transcriptional repression (PubMed:17662997). {ECO:0000269|PubMed:16537907, ECO:0000269|PubMed:17662997, ECO:0000269|PubMed:27181681}.
- Poids moléculaire
- 64.5 kDa
- UniProt
- Q3UZD5
- Pathways
- Regulation of Muscle Cell Differentiation
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