ARHGEF15 Protein (AA 1-849) (Strep Tag)
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- Antigène Voir toutes ARHGEF15 Protéines
- ARHGEF15 (rho Guanine Nucleotide Exchange Factor (GEF) 15 (ARHGEF15))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-849
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ARHGEF15 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MSAQSLPAAT PPTLKPPRII RPRPPSRHRA PHSPGPLHNG SSPKALPQIS NDASASVCTS IFWEPPTASL KPPALLPPSV SRTSLDSQTS PDSPSSTPSP SPVSRRSISP EPAPCSPVPP PKPSGSSRTP LPSGPTPLQD GSASAPGTVR RLAGKFEWGA EGKAQSSDSL ERCSQGSTEV NGEKETPEAA LSGNGSQENG TPDAALACPP CCPCVCHVAK PGLELRWVPV GSSEDILRIP CRASPLRASR SRINPPVISH PPVVLTSYRS TAERKLLPPL KPPKPTKVRQ DISTSEELPQ PDLKLPSEDG IQTATKAWEG DRPEGAPLNA PPVALEGREE EGLDGLKGLQ WELPLQDEPL YQTYRAAVLS EELWGVGEDG GPSPANPGEA PTFSRLPGPR NTLWQELPAV RGSGLLESLS PQERRMQESL FEVVTSEASY LRSLRLLTDT FVLSQALRDT LTPRDHHTLF SNVQRVQSVS ERFLGTLLSR VRSSPHITDL CDVVHAHAVG PFFVYVDYVR NQQYQEETYS RLMDTNVRFS AELRRLQSLP KCERLPLPSF LLLPFQRITR LRMLLQNILS QTEEGSSRQE NAQKALGAVS KIIERCSAEV GRMKQTEELI RLTQRLRFHK VKALPLVSWS RRLELQGELT ELGCRRGGVL FTSRPRFTPL CLLLFSDLLL ITQPKSGQRL QVLDYAHRSL VQAQQVPDPS GPPTFRLSLL SNHQGRPTHR LLQAASLSDM QRWLGAFPTP GPLPCSPDTI YEDCECSQEL CSEPSTPSKT EGQSLESKAP RKHLHKNPEG WLKGLPGAFP AQLVCEVTGE HERRKHLRQH QKLLEAVGPS SGTPDTPQP
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product ARHGEF15 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ARHGEF15 (rho Guanine Nucleotide Exchange Factor (GEF) 15 (ARHGEF15))
- Autre désignation
- Arhgef15 (ARHGEF15 Produits)
- Synonymes
- E5 Protein, ARHGEF15 Protein, ARGEF15 Protein, Ephexin5 Protein, Vsm-RhoGEF Protein, D130071N09 Protein, D530030K12Rik Protein, Rho guanine nucleotide exchange factor 15 Protein, Rho guanine nucleotide exchange factor (GEF) 15 Protein, Arhgef15 Protein, ARHGEF15 Protein
- Sujet
- Rho guanine nucleotide exchange factor 15 (Ephexin-5) (E5),FUNCTION: Specific GEF for RhoA activation. Does not activate RAC1 or CDC42. Regulates vascular smooth muscle contractility. Negatively regulates excitatory synapse development by suppressing the synapse-promoting activity of EPHB2. {ECO:0000269|PubMed:21029865}.
- Poids moléculaire
- 92.9 kDa
- UniProt
- Q5FWH6
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