HCFC1 Protein (AA 2-1081) (His tag)
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- Antigène Voir toutes HCFC1 Protéines
- HCFC1 (Host Cell Factor C1 (VP16-Accessory Protein) (HCFC1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 2-1081
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Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette HCFC1 protéine est marqué à la His tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
- Séquence
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ASAVSPANLP AVLLQPRWKR VVGWSGPVPR PRHGHRAVAI KELIVVFGGG NEGIVDELHV YNTATNQWFI PAVRGDIPPG CAAYGFVCDG TRLLVFGGMV EYGKYSNDLY ELQASRWEWK RLKAKTPKNG PPPCPRLGHS FSLVGNKCYL FGGLANDSED PKNNIPRYLN DLYILELRPG SGVVAWDIPI TYGVLPPPRE SHTAVVYTEK DNKKSKLVIY GGMSGCRLGD LWTLDIETLT WNKPSLSGVA PLPRSLHSAT TIGNKMYVFG GWVPLVMDDV KVATHEKEWK CTNTLACLNL DTMAWETILM DTLEDNIPRA RAGHCAVAIN TRLYIWSGRD GYRKAWNNQV CCKDLWYLET EKPPPPARVQ LVRANTNSLE VSWGAVATAD SYLLQLQKYD IPATAATATS PTPNPVPSVP ANPPKSPAPA AAAPAVQPLT QVGITLVPQA ATAPPSTTTI QVLPTVPGSS ISVPTAARTQ GVPAVLKVTG PQATTGTPLV TMRPASQAGK APVTVTSLPA SVRMVVPTQS AQGTVIGSNP QMSGMAALAA AAAATQKIPP SSAPTVLSVP AGTTIVKTVA VTPGTTTLPA TVKVASSPVM VSNPATRMLK TAAAQVGTSV SSAANTSTRP IITVHKSGTV TVAQQAQVVT TVVGGVTKTI TLVKSPISVP GGSALISNLG KVMSVVQTKP VQTSAVTGQA STGPVTQIIQ TKGPLPAGTI LKLVTSADGK PTTIITTTQA SGAGTKPTIL GISSVSPSTT KPGTTTIIKT IPMSAIITQA GATGVTSSPG IKSPITIITT KVMTSGTGAP AKIITAVPKI ATGHGQQGVT QVVLKGAPGQ PGTILRTVPM GGVRLVTPVT VSAVKPAVTT LVVKGTTGVT TLGTVTGTVS TSLAGAGAHS TSASLATPIT TLGTIATLSS QVINPTAITV SAAQTTLTAA GGLTTPTITM QPVSQPTQVT LITAPSGVEA QPVHDLPVSI LASPTTEQPT ATVTIADSGQ GDVQPGTVTL VCSNPPCETH ETGTTNTATT TVVANLGGHP QPTQVQFVCD RQETAASLVT SAVGQQNGNV VRVCSNPPCE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Hcfc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product HCFC1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- HCFC1 (Host Cell Factor C1 (VP16-Accessory Protein) (HCFC1))
- Autre désignation
- Hcfc1 (HCFC1 Produits)
- Synonymes
- CFF Protein, HCF-1 Protein, HCF1 Protein, HFC1 Protein, MRX3 Protein, VCAF Protein, AW060991 Protein, HCF Protein, RGD1563804 Protein, fc61g09 Protein, hcfc1 Protein, im:7159458 Protein, wu:fc61g09 Protein, wu:fj40c12 Protein, HCFC1 Protein, host cell factor C1 Protein, host cell factor C1a Protein, host cell factor Protein, host cell factor C1 (VP16-accessory protein) Protein, HCFC1 Protein, Hcfc1 Protein, hcfc1a Protein, LOC5571204 Protein, CpipJ_CPIJ014908 Protein
- Sujet
- Involved in control of the cell cycle. Also antagonizes transactivation by ZBTB17 and GABP2, represses ZBTB17 activation of the p15(INK4b) promoter and inhibits its ability to recruit p300. Coactivator for EGR2 and GABP2. Tethers the chromatin modifying Set1/Ash2 histone H3 'Lys-4' methyltransferase (H3K4me) and Sin3 histone deacetylase (HDAC) complexes (involved in the activation and repression of transcription respectively) together. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. {ECO:0000269|PubMed:9334261, ECO:0000269|PubMed:9990006}.
- Poids moléculaire
- 112.1 kDa Including tag.
- UniProt
- Q61191
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