ARHGEF1 Protein (AA 1-920) (Strep Tag)
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- Antigène Voir toutes ARHGEF1 Protéines
- ARHGEF1 (rho Guanine Nucleotide Exchange Factor (GEF) 1 (ARHGEF1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-920
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ARHGEF1 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MGEVAGGAAP GPPRSGLVSI IIGAEDEDFE NELEANSEDQ NSQFQSLEQV KRRPAHLMAL LQHVALQFEP GPLLCCLHAD MLSSLGPKEA KKAFLDFYHS FLEKTAVLRV PVPPSVAFEL DRTRPDLISE DVQRRFIQEV VQSQQAAVSR QLEDFRSKRL MGMTPWEQEL SLLEPWIGKD RGNYEARERH VAERLLSHLE ETQHTISTDE EKSAAVVTAI SLYMRHLGVR TKSGDKKSGR NFFRKKVMGN RRSDEPPKTK KGLSSILDPA RWNRGEPSAP DCRHLKVEAD AEKPGPADRK GGLGMSSRDR TVGTPGQDNP GVSLHPLSTD SVDSREPGVD TPQEPGDTPP QGPTSLEPLA PPESTEDNGE TESPEPGDDG EPGRSGLELE PEEPPGWREL VPPDTLLSLP KSQVKRQEVI SELLVTEAAH VRMLRVLHDL FYQPMADGGF FPLDELQNIF PSLDELIEVH SLFLDRLMKR RQESGYLIEE IGDVLLARFD GAEGSWFQKI SSRFCSRQSF ALEQLKAKQR KEPRFCAFVQ EAESRPRCRR LQLKDMIPTE MQRLTKYPLL LQSIGQNTEE STERGKVELA AECCREILHH VNQAVRDMED LLRLKDYQRR LDLTHLRQSS DPMLSEFKNL DITKKKLVHE GPLTWRVTKD KAIEVHVLLL DDLLLLLQRQ DERLLLKSHS RTLTPTPDGK TMLRPVLRLT SAMTREVATD HKAFYVIFTW DQEAQIYELV AQTSSERKNW CNLITETAGS LKVPAPASRL KPRPSPSSIR EPLLSSSENG TGGAEMAPAD ARTERLLNDL LPFCRPGPEG QLAATALQKV LSLKQILLST EEDSGAGPPR DGDGVPGGRA PGPVHTQEIE ENLLSLEVAI RQLEELEEEF CRLRPLLSQL GGTLSPNLAA PERSAQTGLS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ARHGEF1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ARHGEF1 (rho Guanine Nucleotide Exchange Factor (GEF) 1 (ARHGEF1))
- Autre désignation
- Arhgef1 (ARHGEF1 Produits)
- Synonymes
- arhgef1 Protein, cb473 Protein, zgc:158261 Protein, ARHGEF1 Protein, GEF1 Protein, LBCL2 Protein, LSC Protein, P115-RHOGEF Protein, SUB1.5 Protein, Lbcl2 Protein, Lsc Protein, Rho guanine nucleotide exchange factor (GEF) 1a Protein, Rho guanine nucleotide exchange factor 1 Protein, Rho guanine nucleotide exchange factor (GEF) 1 Protein, arhgef1a Protein, ARHGEF1 Protein, arhgef1 Protein, Arhgef1 Protein
- Sujet
- Rho guanine nucleotide exchange factor 1 (Lbc's second cousin) (Lymphoid blast crisis-like 2),FUNCTION: Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as a GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. Isoform 3 and isoform 4 do not homooligomerize and show an enhanced RhoGEF activity. {ECO:0000269|PubMed:20098430, ECO:0000269|PubMed:8910315}.
- Poids moléculaire
- 102.8 kDa
- UniProt
- Q61210
- Pathways
- Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling
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