SYCP1 Protein (AA 1-993) (Strep Tag)
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- Antigène Voir toutes SYCP1 Protéines
- SYCP1 (Synaptonemal Complex Protein 1 (SYCP1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-993
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette SYCP1 protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), ELISA, SDS-PAGE (SDS)
- Séquence
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MEKQKPFTLF VPPRLSSSQV SAVKPQTAGG DSNYFKTANK CTEGDFGVPF TMSSRENIDK DPAFQKLSIL PMLEQVANSG SCHYQEGVND SDFENSEPMS RLYSKLYKEA EKIKKWKVSI ESELKQKENK LQENRKIIEA QRKAIQELQF ENEKVSLKLE EEIQENKDLI KENNATIHWC NLLKETCARS AEKTNKYEYE REETRQVYVD LNSNIEKMIL AFEELRVQAE NARLEMHFKL KEDHEKIQHL EEEYQKEVNN KENQVSELLI QSAEKENKMK DLTFLLEESR DKANQLEEKT KLQDENLKEL SEKKDHLTSE LEDIKMSMQR SMSTQKALEE DLQIATKTIS QLTEVKEAQM EELNKAKTTH SFVVTELKAT TCTLEELLRT EQQRLEKNED QLKLITVELQ KKSNELEEMT KFKNNKEVEL EELKNILAED QKLLDEKKQV EKLAEELQEK EQELTFLLET REKEVHDLQE QVTVTKTSEQ HYLKQVEEMK TELEKEKLKN TELTASCDML LLENKKFVQE ASDMALELKK HQEDIINCKK QEERLLKQIE NLEEKEMHLR DELESVRKEF IQQGDEVKCK LDKSEENARS IECEVLKKEK QMKILESKCN NLKKQVENKS KNIEELHQEN KTLKKKSSAE IKQLNAYEIK VSKLELELES TKQRFEEMTN NYQKEIENKK ISEGKLLGEV EKAKATVDEA VKLQKEIDLR CQHKIAEMVA LMEKHKHQYD KIVEERDSEL GLYKNREQEQ SSAKIALETE LSNIRNELVS LKKQLEIEKE EKEKLKMAKE NTAILKDKKD KKIQASLLES PEATSWKFDS KTTPSQNISR LSSSMDSGKS KDNRDNLRAS AKSILPTTVT KEYTVKTPTK KSIYQRENKY IPTGGSNKKR KTAFEFDVNS DSSETADLLS LVSEEDVSNR LYDNNPPDSH LLVKTPKQTP LSLSTPASFM KFGSLKKMRE DRWTTIAKID RKRRLKEAEK LFS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product SYCP1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- SYCP1 (Synaptonemal Complex Protein 1 (SYCP1))
- Autre désignation
- Sycp1 (SYCP1 Produits)
- Synonymes
- CT8 Protein, HOM-TES-14 Protein, SCP1 Protein, SYCP1 Protein, T16E15.12 Protein, T16E15_12 Protein, ZYP1 Protein, sycp1 Protein, SCP-1 Protein, syn1 Protein, synaptonemal complex protein 1 Protein, Myosin heavy chain-related protein Protein, SYCP1 Protein, sycp1 Protein, ZYP1a Protein, Sycp1 Protein
- Sujet
- Synaptonemal complex protein 1 (SCP-1),FUNCTION: Major component of the transverse filaments of synaptonemal complexes, formed between homologous chromosomes during meiotic prophase (PubMed:16717126). Required for normal assembly of the central element of the synaptonemal complexes (PubMed:15937223). Required for normal centromere pairing during meiosis (PubMed:22761579). Required for normal meiotic chromosome synapsis during oocyte and spermatocyte development and for normal male and female fertility (PubMed:15937223). {ECO:0000269|PubMed:15937223, ECO:0000269|PubMed:16717126, ECO:0000269|PubMed:22761579}.
- Poids moléculaire
- 115.9 kDa
- UniProt
- Q62209
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