BTBD12 Protein (AA 1-1565) (Strep Tag)
-
- Antigène Tous les produits BTBD12
- BTBD12 (BTB (POZ) Domain Containing 12 (BTBD12))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1565
- Origine
- Souris
-
Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette BTBD12 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
-
MVPESAPNGN SQPLPSCFTT TGVPSPSKPR VSELVLQRMK QFKRADPERL RHASEESPQK TALGDDVPRS PPEETVGENE YKLDATDSDA AMALALQQEF RREEASSHHD SLEEKGLFFC QMCQKNLSAM NVTRREQHVN RCLDEAEKAQ RPASPRIPDC PICGKPFLTT KSRISHLKQC AVRMEVGPQL LLQAVRLQTA QPEVDGSPQV PSFSNNVGGL KRKGVTTKRE PRRRKVNKPE APSEDLLVAM ALSRSEVEHC PVVPPLRLEN AFSEKIRLGA EKKSRKKRPP VCPPQLVTQD SETTGRQIED RVAQLLSEEA ELSCTPPLLA SKISKEELEP AGWRARLPEG KRNFLWELSA LTGAWAEESF YTVGLFPPIV SQCPSKEPQL PLELPKQGEP SPRRPPASQS SLPVSHSPKI RLLSSSQRER QALQDLVDLA VEGLSSSPQP GSRGVPTGLD LVPSSLPLTG FVLPCKKTLK KDDSASLSLG LLVTDFGAMV NNPHLSDVQF QLDSGEVLYA HKFVLYARCP LLIQYVSTES FSSEEDGDLT QRALLSDVSS EAAHAFLNYL YMADTDMPPS LVPDLRSLAL RFGVSDLVQL CEQVPAVVDL EGEQPEETSE DCESRAETFL ELLRSVWVDN EEEVETLLKP ELCEEERERV NEAEMEEIYE FAATQRKLLQ WGRAADPDGS TNPHGEDGAV SEPSLAGVQS NRQLENTEHM ESSGLEKEEA LASWEQEGHS TPLQDQCPDW AGKAEAQDAL GEATDDPSFC SRHRRGKECL PLHPNKAHGC KQPLPSNPRV SSELSQITVD HEEQSDHVRE TQADMAQAPT PHSCSLVSQS SVDGSPSQSW LHLYHTSHLS PSVSQSHSSI SRVASPRSLS PTTPTKQRRG SNIVTLRKDA GHHRGQQSSP IAGHRNRGIL ISPAKSPPID LTQSVPEPLS PRAQDPLHFV KKEDEVILLL DSDEELEHTK TESVSKDSPE GRKVPEFSPR SSELFSVIDV EEDHEHFQSP LKREAGLQHG EEGQLGNQSA LGCRDIPWLL CSQKTSLDED SATDTSWLVP ATPGVSRSRD CSSQTQIKSL KTRIPSDETA QQTPRPNLER RTMLETAQQF SVIMPHTQPI TLGAFDSGRQ AYRSPSHPYP RHHRLSSSQP SCPGPDFTRW SQKSSAPRPC LPNLPAADDV VEVGDSDDEV ASHQGNSSPV LDGDPPGPMG DYCWNEPLSP IPIDHLNLER TGPLTTSSPS SQVLEALHSD DCHSPGLGTT PIRGSCGTLR ESQERSSLAG SPEALWDDWN EEEGQSPEAP PVAQMLSTRT RKPDRPETPK GANQKKNLPP KVPITPMPRY SIMETPVLKK ELDRFGVRAL PKRQMVLKLK EIFQYTHQTL ESDSEDEVQS PQIPAELPCR QASTTETCNP SRLPTGEPSH PDGDAQLPAS QESMATSVDG SDNSFSSKSS SAEFGAAFEY SDEDKDEEVG VTASQAAIQA ADTEEAVRRY IRSKPALHRQ VLRYQPVELA ELQAELKQNG IPVAMGKLSD ILDAQCITFT TAAARKEKLK HKRRQPSGRK KKDQK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- BTBD12 (BTB (POZ) Domain Containing 12 (BTBD12))
- Autre désignation
- Slx4 (BTBD12 Produits)
- Synonymes
- Btbd12 Protein, RGD1566009 Protein, BTBD12 Protein, FANCP Protein, MUS312 Protein, AI256635 Protein, AI426760 Protein, D16Bwg1016e Protein, SLX4 structure-specific endonuclease subunit Protein, SLX4 structure-specific endonuclease subunit homolog Protein, SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae) Protein, Slx4 Protein, SLX4 Protein
- Sujet
- Structure-specific endonuclease subunit SLX4 (BTB/POZ domain-containing protein 12),FUNCTION: Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks (By similarity). {ECO:0000250}.
- Poids moléculaire
- 172.4 kDa
- UniProt
- Q6P1D7
- Pathways
- Réparation de l'ADN
-