CEP135 Protein (AA 1-1140) (Strep Tag)
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- Antigène Tous les produits CEP135
- CEP135 (Centrosomal Protein 135kDa (CEP135))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1140
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette CEP135 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MTTAAERKYI NIRKRLDQLG YRQTLSVDSL PLVEKLFSDL VHTTESLRQC RLSSGKAEKE SANLDFVLEP YKLENTRLNK ENNELYLELM KLRECSDKHI KDLKTTLKKC SRETADLKFL NNQYVHKVKV LEKESKAKDE KIQQLQEKNL RAVVQTPGGR KRNIAFRRQR MQIDEPAPPS EVSAYPVPQP EDPYIADLLQ VADNRIQELQ EEVQQLQEKL AQMEKGVLDY SKQIELRERE IQRLSLALDG GCSPDVLSLE TRNKTNEKLI AHLNVQVDFL QQANKELEKH IQELMETKET VTTEVVNLSN RNEKLCQELT EIDQLAQRLE RHKEQVLETA DKELGEAKKE IKRNLCEMRN LEEKMSKLQW ELDLSHKEKE RLNSELLLKS DLETVVHQLE QEKQRLSKKL QSFAVTEREL TLEVERMRLE HGIKRRDKSP SRLDTFLKGI EEERDYYKKE LEKLQHLIQR RSCAINYSAR EKPPVVKCSE KGDCSTDVHL ITRERDELQR MLERFEKYME DIQSNVKLLT AERDKLNVLY KEAKEELSTL RKESTNSTSP NHLVSCVEKE KERALSELRR ITAEKEALRE KLKNIQERNA VGKSDLEKTI EHLTYINHQL ENEKYELQSK MLMMKETVES LENKSKLQAQ KLSHVTGDSS HQKTEMTSLR IVSEQLQRSL DDCQHRLSIK RGELESAQEQ IKMLEQKLEN LSHRMTVQSE ETHAMKKTIG VMDKEKDFLQ ETVDEKTEKI ANLQESLLSK EKVIAQLKVT VAEYETSLNQ LQETLTTRDR EINSLRRQLD ASHKELDDVG KSREISFKEN RRLQDDLATM ARENQEISLE LEAAVQEKEE MKSRVHKYIT EVSRWESLMA AKEKENKDLL DRFQMLHSRA EDWEVKAQQA EGENSSVRLE LLSIDTERRH LRERVDLLEK EIQEHINAHH AYESQISSMA KAMSQLEEEL RRHESEKATM LGDVSSLREL CIKLDSGKDV MTQQLNSKSL ELERAVAELE NVKSESELLK KQLTNERQTI KNLESLLATN RDKEFQSHLT SHEKDTEIQL LKEKLNLSES KLTTQSRETS MLRTKVTQLQ TDYDNLKRQM SNEKYERERA IQEMRRLGLP TSPLSSTLKS PVQTPDHINA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- CEP135 (Centrosomal Protein 135kDa (CEP135))
- Autre désignation
- Cep135 (CEP135 Produits)
- Synonymes
- RGD1310726 Protein, BC062951 Protein, Cep4 Protein, Gm1044 Protein, CEP4 Protein, KIAA0635 Protein, MCPH8 Protein, centrosomal protein 135 Protein, centrosomal protein 135kDa Protein, centrosomal protein 135kDa L homeolog Protein, centrosomal protein of 135 kDa Protein, CEP135 Protein, cep135 Protein, cep135.L Protein, LOC100455863 Protein, Cep135 Protein
- Sujet
- Centrosomal protein of 135 kDa (Cep135) (Centrosomal protein 4),FUNCTION: Centrosomal microtubule-binding protein involved in centriole biogenesis. Acts as a scaffolding protein during early centriole biogenesis. Required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. Also required for centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Required for the recruitment of CEP295 to the proximal end of new-born centrioles at the centriolar microtubule wall during early S phase in a PLK4-dependent manner (By similarity). {ECO:0000250|UniProtKB:Q66GS9}.
- Poids moléculaire
- 133.3 kDa
- UniProt
- Q6P5D4
- Pathways
- M Phase, SARS-CoV-2 Protein Interactome
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