INTS6 Protein (AA 1-883) (Strep Tag)
-
- Antigène Voir toutes INTS6 Protéines
- INTS6 (Integrator Complex Subunit 6 (INTS6))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-883
- Origine
- Souris
-
Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette INTS6 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
-
MPILLFLIDT SASMNQRSHL GTTYLDTAKG AVETFMKLRA RDPASRGDRY MLVTFEEPPY AIKAGWKENH ATFMNELKNL QAEGLTTLGQ SLRTAFDLLN LNRLVTGIDN YGQGRNPFFL EPAIIITITD GSKLTTTSGV QDELHLPLNS PLPGSELTKE PFRWDQRLFA LVLRLPGTMS VESEQLTGVP LDDSAITPMC EVTGGRSYSV CSPRMLNQCL ESLVQKVQSG VVINFEKAGP DPPPAEEGQP DISRPFGSQP WHSCHKLIYV RPNPKTGVPI GHWPVPESFW PDQNSPTLPP RTSHPVVKFS CTDCEPMVID KLPFDKYELE PSPLTQFILE RKSPQTCWQV YVSNSAKYNE LGHPFGYLKA STALTCVNLF VMPYNYPVLL PLLDDLFKVH KAKPTLKWRQ SFESYLKTMP PYYLGPLKKA VRMMGAPNLI ADSMEYGLSY SVISYLKKLS QQAKIESDRV IGSVGKKVVQ ETGIKVRSRS HGLSMAHRKG FQVLQGISED VPHRLLDLNM KEYTGFQVAL LNKDLKPQTF RNAYDIPRRN LLDHLTRMRS NLLKSTRKFL KGQDEDQVHS VPIAQMGNYQ EYLKQVPSPL RELDPDQPRR LHTFGNPFKL DKKGMMIDEA DEFVAGPQNK HKRPGEPSMQ GIPKRRRCAS PLLRGRRQSP AVNSHIGGKG PPAPMTQAQP GLIKPLPLHK EATNDSIVDD VVENHVADQL SSDMTPNAMD TEFLTSPPNL LEPSTNHTEA LGHEHLGNND LTVGGFLENH EEPRNKEQSA EENIPASSLN KGKKLMHCRS HEEVNTELKA QIMKEIRKPG RKYERIFTLL KHVQGSLQTR LIFLQNVIKE ASRFKKRMLI EQLENFLDEI HRRANQINHI NSN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
-
Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
-
Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product INTS6 Protéine
-
-
- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
-
ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
-
- Antigène
- INTS6 (Integrator Complex Subunit 6 (INTS6))
- Autre désignation
- Ints6 (INTS6 Produits)
- Synonymes
- DBI-1 Protein, DDX26 Protein, DDX26A Protein, DICE1 Protein, HDB Protein, INT6 Protein, Notchl2 Protein, EIF3-P48 Protein, EIF3S6 Protein, eIF3-p46 Protein, 2900075H24Rik Protein, AI480962 Protein, Ddx26 Protein, Notch2l Protein, LRRGT00024 Protein, ints6 Protein, zgc:63527 Protein, INTS6 Protein, ints6-b Protein, Int6-A Protein, dbi-1 Protein, ddx26 Protein, ddx26a Protein, dice1 Protein, hdb Protein, int6 Protein, ints6-a Protein, notchl2 Protein, integrator complex subunit 6 Protein, eukaryotic translation initiation factor 3 subunit E Protein, integrator complex subunit 6 like Protein, integrator complex subunit 6 S homeolog Protein, integrator complex subunit 6 L homeolog Protein, INTS6 Protein, EIF3E Protein, Ints6 Protein, ints6l Protein, ints6 Protein, ints6.S Protein, Tsp_09465 Protein, LOC100164318 Protein, ints6.L Protein
- Sujet
- Integrator complex subunit 6 (Int6) (DBI-1) (Protein DDX26),FUNCTION: Component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Mediates recruitment of cytoplasmic dynein to the nuclear envelope, probably as component of the INT complex. {ECO:0000250|UniProtKB:Q9UL03}.
- Poids moléculaire
- 99.7 kDa
- UniProt
- Q6PCM2
-