DHX29 Protein (AA 1-1365) (Strep Tag)
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- Antigène Voir toutes DHX29 Protéines
- DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1365
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette DHX29 protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), ELISA, SDS-PAGE (SDS)
- Séquence
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MGGKNKKHKA PGAAAMRAAV SASRARSAEA GAVGEAQSKK PVARPAPAVP TGAREPRVKQ GPKIYSFNSA NDSGGSANLD KSILKVVINN KLEQRIIGVI NEHKKQNSDR GAISGRLSAK KLQDLYMALQ AFSFKTKDIE DAMTNTLLHG GDLHSALDWL CLNLSDDALP EGFSQEFEEQ QPKSRPKFQS VQIQATLSPP QQTKTKRQEE DPKIKPKKEE TTVEVNMKEW ILRYAEQQDE EEKGEGSKGL EEEEKFDPNQ RYLNLAARLL DAKEQAAAFK LEKNKQGQKE AQEKIRKFQR EMETLEDHPI FNPAIKISHQ QNEKKKPAPA TEAESALNLN LFEKSAAATE EEKGKKKEPH DVRNFDYTAR SWTGKSPKQF LIDWVRKNLP KSPNPSFEKV AVGRYWKCRV RVVRSEDDVL VVCPTILTED GMQAQHLGAT LALYRLVKGQ SVHQLLPPTY RDVWLEWSDE EKKREELNKM ETNKPRDLFI AKLLNKLKQQ QQQQQQQRPE SEKGGSEDPE ESWENLVSDE DLAALSLEPT SAEDLAPVRS LFRRLQSTPK YQRLLKERQQ LPVFKHRDSI VETLKRHRVV VVAGETGSGK STQVPHFLLE DLLLDECGAR KCNIVCTQPR RISAVSLATR VCEELGCESG PGGRNSLCGY QIRMESRASE STRLLYCTTG VLLRKLQEDG LLADVSHVIV DEVHERSVQS DFLLVILKEI LQKRSDLHLI LMSATVDSDK FSTYFTHCPI LRISGRSYPV EVFHLEDIVE ETGFVLEKDS EYCQKFLEEE EEITINVTSK AGGVKKYQEY IPVQSGASPE LNPFYQKYSS RTQHAILYMN PHKINLDLIL ELLVYLDKSP QFRNIEGAVL IFLPGLAHIQ QLYDLLSSDR RFYSERYQVI ALHSVLSTQD QAAAFMFPPP GVRKIVLATN IAETGITIPD VVFVIDTGRT KENKYHESSQ MSSLVETFVS KASALQRQGR AGRVRDGFCF RLYTRERFEG FLDYSVPEIL RVPLEELCLH IMKCDLGSPE DFLSKALDPP QLQVISNAMN LLRKIGACEP NEPKLTPLGQ HLAALPVNVK IGKMLIFGAI FGCLEPVATL AAVMTEKSPF ITPIGRKDEA DLAKSSLAVA DSDHLTIYNA YLGWKKAQQE GGFRSEISYC QRNFLNRTSL LTLEDVKQEL MKLVKAAGFS SSPSWEGRKG PQTLSFQDIA LLKAVLAAGL YDSVGKIMCT KSVDVTEKLA CMVETAQGKA QVHPSSVNRD LQTYGWLLYQ EKVRYTRVYL RETTLITPFP VLLFGGDIEV QHRERLLSVD GWIYFQAPVK IAVIFKQLRV LIDSVLRKKL ENPKMSLEND KILQIITELI KTENN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- DHX29 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 29 (DHX29))
- Autre désignation
- Dhx29 (DHX29 Produits)
- Synonymes
- DDX29 Protein, 3732415M03 Protein, AU043276 Protein, Ddxx Protein, E130202M19Rik Protein, DExH-box helicase 29 Protein, DEAH (Asp-Glu-Ala-His) box polypeptide 29 Protein, DEAH-box helicase 29 L homeolog Protein, DEAH-box helicase 29 Protein, DHX29 Protein, Dhx29 Protein, dhx29.L Protein, dhx29 Protein
- Sujet
- ATP-dependent RNA helicase DHX29 (EC 3.6.4.13) (DEAH box protein 29),FUNCTION: ATP-binding RNA helicase involved in translation initiation. Part of the 43S pre-initiation complex that is required for efficient initiation on mRNAs of higher eukaryotes with structured 5'-UTRs by promoting efficient NTPase-dependent 48S complex formation. Specifically binds to the 40S ribosome near the mRNA entrance. Does not possess a processive helicase activity. {ECO:0000255|HAMAP-Rule:MF_03068}.
- Poids moléculaire
- 154.0 kDa
- UniProt
- Q6PGC1
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