INO80 Protein (AA 1-1559) (Strep Tag)
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- Antigène Tous les produits INO80
- INO80 (INO80 Homolog (INO80))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1559
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette INO80 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MASELGAGDD GSSTELAKPL YLQYLERALR LDHFLRQTSA IFNRNISSDD SEDGLDDNNP LLPESGDPLI QVKEEPPNSL LGETSGASSS GLLNPYSLNG VLQSESKSDK GNLYNFSKLK KSRKWLKSIL LSDESSEADS QSEDNDDEEE ELSLSREELH NMLRLHKYKK LHQNKYSKDK ELQQYQYYSA GLLSTYDPFY EQQRHLLGPK KKKFKEDKKL KAKLKKVKKK RRRDEEFSSE ESPRHHHHQT KVFAKFSHDA PPPGTKKKHL SIEQLNARRR KVWLSIVKKE LPKANKQKSS ARNLFLTNSR KLAHQCMKEV RRAALQAQKN CKETLPRARR LTKEMLLYWK KYEKVEKEHR KRAEKEALEQ RKLDEEMREA KRQQRKLNFL ITQTELYAHF MSRKRDMGHD GIQEEILRKL EDSSTQRQID IGGGVVVNIT QEDYDSNHFK AQALKNAENA YHIHQARTRS FDEDAKESRA AALRAADKSG SGFGESYSLA NPSIRAGEDI PQPTIFNGKL KGYQLKGMNW LANLYEQGIN GILADEMGLG KTVQSIALLA HLAERENIWG PFLIISPAST LNNWHQEFTR FVPKFKVLPY WGNPHDRKVI RRFWSQKTLY TQDAPFHVVI TSYQLVVQDV KYFQRVKWQY MVLDEAQALK SSSSVRWKIL LQFQCRNRLL LTGTPIQNTM AELWALLHFI MPTLFDSHEE FNEWFSKDIE SHAENKSAID ENQLSRLHMI LKPFMLRRIK KDVENELSDK IEILTYCQLT SRQKLLYQAL KNKISIEDLL QSSMGSTQQA QNTTSSLMNL VMQFRKVCNH PELFERQETW SPFHISLKPY EISKFIYRHG QIRVFNHSRD RWLKVLLSPF APDYIQQSLF HRKGINEGSC FSFLRFIDVS PAEMANLMLQ GLLARWLALF LSLKASYRLH QLRSWAEPDG TSHQSYLRNK DFLLGVDFPL SFPNLCSCPL LKSLVFSSHC KAVSGYSDHV VHQRRSATSS LRCCLLTELP SFLCVASPRV TAVPLDSYCN DRSAEYERGV LKEGGSLAAK QCLLNGAPEL ATDWLSRRSQ FFPEPAGGLL SIRPQNGWSF IRIPGKESLI TDSGKLYALD VLLTRLKSQG HRVLIYSQMT RMIDLLEEYM VYRKHTYMRL DGSSKISERR DMVADFQTRN DIFVFLLSTR AGGLGINLTA ADTVIFYDSD WNPTVDQQAM DRAHRLGQTK QVTVYRLICK GTIEERILQR AKEKSEIQRM VISGGNFKPD TLKPKEVVSL LLDDEELEKK LRLRQEEKRQ QEESNRVKER KRKREKYAEK KKKEDELDGK RRKEGVNLVI PFVPSADNSN LSADGDDSFI SVDSAMPSPF SEISISSELH TGSIPPDESS SDMLVIVDDP ASSAPQSRAT NSPASITGSV SDTVNGISIQ EVPAAGRGHS ARSRGRPKGS GSTAKGAGKG RSRKSTAGSA AAMAGAKAGA AAASAAAYAA YGYNVSKGIS ASSPLQTSIV RPAGLADFGP SSASSPLSSP LNKGNNIPGT PKSLHMTSSL ASDSLIRKQG KGTNPSGGR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- INO80 (INO80 Homolog (INO80))
- Autre désignation
- Ino80 (INO80 Produits)
- Synonymes
- ATINO80 Protein, INO80 ORTHOLOG Protein, INO80 ortholog Protein, INO80A Protein, INOC1 Protein, hINO80 Protein, Inoc1 Protein, RGD1310969 Protein, 2310079N15Rik Protein, 4632409L19Rik Protein, DNA helicase INO80-like protein Protein, INO80 complex subunit Protein, INO80 Protein, Ino80 Protein
- Sujet
- Chromatin-remodeling ATPase INO80 (EC 3.6.4.-) (DNA helicase-related INO80 complex homolog 1) (DNA helicase-related protein INO80),FUNCTION: ATPase component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and DNA repair. Binds DNA. As part of the INO80 complex, remodels chromatin by shifting nucleosomes. Regulates transcription upon recruitment by YY1 to YY1-activated genes, where it acts as an essential coactivator. Involved in UV-damage excision DNA repair. The contribution to DNA double-strand break repair appears to be largely indirect through transcriptional regulation. Involved in DNA replication. Required for microtubule assembly during mitosis thereby regulating chromosome segregation cycle. {ECO:0000250|UniProtKB:Q9ULG1}.
- Poids moléculaire
- 176.5 kDa
- UniProt
- Q6ZPV2
- Pathways
- Positive Regulation of Response to DNA Damage Stimulus
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