C2CD5/KIAA0528 Protein (AA 1-1016) (Strep Tag)
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- Antigène Tous les produits C2CD5/KIAA0528 (C2CD5)
- C2CD5/KIAA0528 (C2CD5) (C2 Calcium Dependent Domain Containing 5 (C2CD5))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1016
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette C2CD5/KIAA0528 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MPGKLKVKIV AGRHLPVMDR ASDLTDAFVE VKFGNTTFKT DVYLKSLNPQ WNSEWFKFEV DDEDLQDEPL QITVLDHDTY SANDAIGKVY IDIDPLLYSE AATVISGWFP IYDTIHGIRG EINVVVKVDL FNDSNRFRQS SCGVKFFCTT SIPKCYRAVV IHGFVEELVV NEDPEYQWID RIRTPRASNE ARQRLISLMS GELQRKIGLK VLEMRGNAVV GYLQCFDLEG ESGLVVRAIG TACTLDKLSS PAAFLPACSS PSRELKEIPF NEDPNPNTHS SGPSTPLKNQ TYSFSPSKSY SRQSSSSDTD LSLTPKTGMG SGSAGKEGGP FKALLRQQTQ SALEQREFPF LTLTAFPPGL LVHVGGVVSA RSVKLLDRIH NPDEPETRDA WWAEIRQEIK SHAKALGCHA VVGYSESTSI CEEVCILSAS GTAAVLNPRF LQEGTVEGCL EQRIEENLPV GCGFCHIPYD ELNMPFPAHL TYCYNCRKQK VPDVLFTTID LPTDAVVVGK GCLIQARLCR LKKKAQAEAN ATAISNLLPF MEYEVHTQLM NKLKLKGMNA LFGLRIQITV GETMLMGLAS ATGVYLAALP TPGGIQIAGK TPNDGSYEQH ISHMQKRIND TIAKNKELYE ITPPEVSEEM IGSPIPEPRQ RSRLLRSQSE SSDEVTELDL SHGKKDAFVL EIDDTDAMED VHSLLTDAPP PSGFYSCNTE IMPGINNWTS EIQMFTSVRV VRLSSLNLTN QALNKNFNGL CENLLKSLYF KLRSMTPCCL CHVNFTVSLP EDELIQVTVT AVAITFDKNQ ALQTTKPHVE KSLQRASTDN EELLQFPLEL CSDSLPPHPF PAAKEHLESA NSNSGIPAAQ RAVTVEKASA MGDGNFRNRS APPCASPTVG VVKMTPLSFI PGAKITKYLG IINMFFIRET TSLREEGGVS GFLHAFIAEV FAMVRAHVAA LGGNAVVSYI MKQCVFMENP SKNQAQCLIN VSGDAVVFVR DSDLEVMSSQ QPAANCQPSC TGEVTT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- C2CD5/KIAA0528 (C2CD5) (C2 Calcium Dependent Domain Containing 5 (C2CD5))
- Autre désignation
- C2cd5 (C2CD5 Produits)
- Synonymes
- CDP138 Protein, KIAA0528 Protein, 5730419I09Rik Protein, C030008B15Rik Protein, Kiaa0528 Protein, kiaa0528 Protein, CH1073-429G16.1 Protein, fj43c12 Protein, si:ch1073-429g16.1 Protein, si:ch73-30n17.4 Protein, wu:fj43c12 Protein, zgc:63659 Protein, zgc:92130 Protein, RGD1304592 Protein, C2 calcium dependent domain containing 5 Protein, C2 calcium-dependent domain containing 5 Protein, C2 calcium-dependent domain containing 5 L homeolog Protein, KIAA0528 ortholog Protein, C2CD5 Protein, C2cd5 Protein, c2cd5.L Protein, c2cd5 Protein, KIAA0528 Protein
- Sujet
- C2 domain-containing protein 5 (138 kDa C2 domain-containing phosphoprotein),FUNCTION: Required for insulin-stimulated glucose transport and glucose transporter SLC2A4/GLUT4 translocation from intracellular glucose storage vesicle (GSV) to the plasma membrane (PM) in adipocytes. Binds phospholipid membranes in a calcium-dependent manner and is necessary for the optimal membrane fusion between SLC2A4/GLUT4 GSV and the PM. {ECO:0000269|PubMed:21907143}.
- Poids moléculaire
- 111.7 kDa
- UniProt
- Q7TPS5
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