ZBTB33 Protein (AA 1-671) (Strep Tag)
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- Antigène Voir toutes ZBTB33 Protéines
- ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-671
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette ZBTB33 protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MESRKLISAT DIQYSASLLN SLNEQRGHGL FCDVTVIVED RKFRAHRNIL SASSTYFHQL FSVAGQVVEL SFIRAEIFAE ILNYIYSSKV VRVRADLLDE LIKSGQLLGV KFIAELGVPL SQVKSISGTE QDGTAETLPS SSSDKSLDME KSKDEAQDNG ATVMPIITES FSLSAEDNEM KKIIVTDSDD DDDDDVIFCS EILPAKEDLP SNNTATQVQP NPASVAISEV TPCASNNSPP VTNITPTQLP TPVNQATLSQ TQGSEELLVS SASTHLTPNI ILLNQAPLTA PPSASSSLPN HMSSSVNVLV QNQQTPNSAV LTGNKAEEEE EIIDDDDDII SSSPDSAVSN TSLVPQADNS KSTTLDGSLT QKMQIPVLPQ EPPSNSLKIS DVITRNTNDP GLRSKHVMEG QKIITLDTAT EIEGLSTGCK VYANIGEDTY DIVIPVKDDP DGGEAKLDNE LPKTSGSEPP NKRMKVKHDD HYELIVDGRV YYICIVCKRS YVCLTSLRRH FNIHSWEKKY QCRYCDKVFP LAEYRTKHEI HHTGERRYQC LTCGKSFINY QFMSSHIKSV HSQDPSGDSK LYRLHPCKSL QIRQYAYLSN KSSAMPVMKD DAVGYKVDAG KEPPVGTTST PPQNKSTFWE DIFIQQENDS IFKQNVTDGS TEFEFIIPES Y
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product ZBTB33 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
- Autre désignation
- Zbtb33 (ZBTB33 Produits)
- Synonymes
- kaiso Protein, ZNF-kaiso Protein, ZNF348 Protein, AW260253 Protein, E130014G12Rik Protein, Kaiso Protein, Znf-kaiso Protein, RGD1566309 Protein, zinc finger and BTB domain containing 33 S homeolog Protein, zinc finger and BTB domain containing 33 Protein, zbtb33.S Protein, ZBTB33 Protein, Zbtb33 Protein
- Sujet
- Transcriptional regulator Kaiso (Zinc finger and BTB domain-containing protein 33),FUNCTION: Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). May recruit the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. Contributes to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (By similarity). {ECO:0000250|UniProtKB:Q86T24, ECO:0000269|PubMed:15138284, ECO:0000269|PubMed:15282317, ECO:0000269|PubMed:15564377, ECO:0000269|PubMed:15817151}.
- Poids moléculaire
- 74.1 kDa
- UniProt
- Q8BN78
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