Importin 11 Protein (IPO11) (AA 1-975) (Strep Tag)
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- Antigène Voir toutes Importin 11 (IPO11) Protéines
- Importin 11 (IPO11)
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-975
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette Importin 11 protéine est marqué à la Strep Tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), ELISA
- Séquence
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MDLNSASSVV LQVLTQATSQ DTAVLKPAEE QLKQWETQPG FYSVLLNIFT NHTLDINVRW LAVLYFKHGI DRYWRRVAPH ALSEEEKSTL RAGLITNFNE PINQIATQIA VLIAKVARLD CPRQWPELIP TLVESVKVQD DLRQHRALLT FYHVTKTLAS KRLAADRKLF YDLASGIYNF ACSLWNHHTD TFLQHVSSGN EAAVLSSLER TLLSLKVLRK LTVNGFVEPH KNMEVMGFLH GIFERLKQFL ECSRSIGTDN VCRDRLEKTI ILFTKVLLDF LDQHPISFTP LIQRSLEFSV SYVFTEVGEG VTFERFIVQC MNLIKMIVKN YAYKPSKNFE DSSPETLEAH KIKMAFFTYP TLTEICRRLV SHYFLLTEEE LTMWEEDPEG FTVEETGGDS WKYSLRPCTE VLFIDIFHEY NQTLTPVLLE MMQTLEGPTN VEDMNALLIK DAVYNAVGLA AFELFDSVDF DQWFKTQLLP ELQVSHNRYK PLRRRVIWLI GQWISVKFKS DLRPMLYEAI CNLLQDQDLV VRIETATTLK LTVDDFEFRT DQFLPYLETM FTLLFQLLQQ VTECDTKMHV LHVLSCVIER VNVQIRPYVG CLVQYLPLLW KQSEEHNMLR CAILTTLIHL VQGLGADSKN LYPFLLPVIQ LSTDVSQPPH VYLLEDGLEL WLVTLENSPC VTPELLRIFQ NMSPLLELSS ENLRTCFKII NGYIFLSSTE FLQTYAAGLC QSFYELLPEI TTEGQVQVLK VVENALKVNP VLGPQMFQRI LPCVFRGVIE GERYPVVMSI YLAVMGRVLL QNTSFFSSLL NEMGHEFNQE MDQLLGNVIE MWVDRMDNIT QPERKKLSAL ALLSLLPSDN SVIQDKFCGI INISVEALHD VMTEDPETRT YKDCMLMSQH EEPKVTEDEE PPTEQDKRKK MLALKDPVHT VSLQQFIYEK LKAQQEILGE QGFQSLMETV DTEIVTQLQE FLQGF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Top Product
- Discover our top product IPO11 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Importin 11 (IPO11)
- Autre désignation
- Ipo11 (IPO11 Produits)
- Synonymes
- MGC83107 Protein, IPO11 Protein, slrn Protein, ranbp11 Protein, RanBP11 Protein, 1700081H05Rik Protein, 2510001A17Rik Protein, AI314624 Protein, AW555235 Protein, E330021B14Rik Protein, Ranbp11 Protein, importin 11 S homeolog Protein, importin 11 Protein, importin-11 Protein, ipo11.S Protein, ipo11 Protein, IPO11 Protein, AFUA_1G03590 Protein, BDBG_04626 Protein, LOC100162908 Protein, Ipo11 Protein
- Sujet
- Importin-11 (Imp11) (Ran-binding protein 11) (RanBP11),FUNCTION: Functions in nuclear protein import as nuclear transport receptor. Serves as receptor for nuclear localization signals (NLS) in cargo substrates. Is thought to mediate docking of the importin/substrate complex to the nuclear pore complex (NPC) through binding to nucleoporin and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to the importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (By similarity). Mediates the nuclear import of RPL12, and of UBE2E3 (By similarity). {ECO:0000250, ECO:0000269|PubMed:11809816}.
- Poids moléculaire
- 112.4 kDa
- UniProt
- Q8K2V6
- Pathways
- Protein targeting to Nucleus
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