BCL11B Protein (AA 1-884) (Strep Tag)
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- Antigène Tous les produits BCL11B
- BCL11B (B-Cell CLL/lymphoma 11B (Zinc Finger Protein) (BCL11B))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-884
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette BCL11B protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MSRRKQGNPQ HLSQRELITP EADHVEATIL EEDEGLEIEE PSSLGLMVGG PDPDLLTCGQ CQMNFPLGDI LVFIEHKKKQ CGGLGPCYDK VLDKSSPPPS SRSELRRVSE PVEIGIQVTP DEDDHLLSPT KGICPKQENI AGPCRPAQLP SMAPIAASSS HPPTSVITSP LRALGVLPPC FPLPCCGARP ISGDGTQGEG QMEAPFGCQC ELSGKDEPSS YICTTCKQPF NSAWFLLQHA QNTHGFRIYL EPGPASTSLT PRLTIPPPLG PETVAQSPLM NFLGDSNPFN LLRMTGPILR DHPGFGEGRL PGTPPLFSPP PRHHLDPHRL SAEEMGLVAQ HPSAFDRVMR LNPMAIDSPA MDFSRRLREL AGNSSTPPPV SPGRGNPMHR LLNPFQPSPK SPFLSTPPLP PMPAGTPPPQ PPAKSKSCEF CGKTFKFQSN LIVHRRSHTG EKPYKCQLCD HACSQASKLK RHMKTHMHKA GSLAGRSDDG LSAASSPEPG TSELPGDLKA ADGDFRHHES DPSLGPEPED DEDEEEEEEE LLLENESRPE SSFSMDSELG RGRENGGGVP PGVAGAGAAA AALADEKALA LGKVMEDAGL GALPQYGEKR GAFLKRAGDT GDAGAVGCGD AGAPGAVNGR GGAFAPGAEP FPALFPRKPA PLPSPGLGGP ALHAAKRIKV EKDLELPPAA LIPSENVYSQ WLVGYAASRH FMKDPFLGFT DARQSPFATS SEHSSENGSL RFSTPPGDLL DGGLSGRSGT ASGGSTPHLG GPGPGRPSSK EGRRSDTCEY CGKVFKNCSN LTVHRRSHTG ERPYKCELCN YACAQSSKLT RHMKTHGQIG KEVYRCDICQ MPFSVYSTLE KHMKKWHGEH LLTNDVKIEQ AERS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- BCL11B (B-Cell CLL/lymphoma 11B (Zinc Finger Protein) (BCL11B))
- Autre désignation
- Bcl11b (BCL11B Produits)
- Synonymes
- BCL11B Protein, 9130430L19Rik Protein, AI604821 Protein, B630002E05Rik Protein, BCL-11B Protein, Ctip2 Protein, Rit1 Protein, ATL1 Protein, ATL1-alpha Protein, ATL1-beta Protein, ATL1-delta Protein, ATL1-gamma Protein, CTIP-2 Protein, CTIP2 Protein, RIT1 Protein, ZNF856B Protein, hRIT1-alpha Protein, B-cell CLL/lymphoma 11B Protein, B cell leukemia/lymphoma 11B Protein, B-cell CLL/lymphoma 11B (zinc finger protein) Protein, BCL11B Protein, Bcl11b Protein
- Sujet
- B-cell lymphoma/leukemia 11B (BCL-11B) (B-cell CLL/lymphoma 11B) (COUP-TF-interacting protein 2) (Radiation-induced tumor suppressor gene 1 protein) (mRit1),FUNCTION: Key regulator of both differentiation and survival of T-lymphocytes during thymocyte development in mammals (PubMed:12717433). Essential in controlling the responsiveness of hematopoietic stem cells to chemotactic signals by modulating the expression of receptors CCR7 and CCR9, which direct the movement of progenitor cells from the bone marrow to the thymus (By similarity). Is a regulator of IL2 promoter and enhances IL2 expression in activated CD4(+) T-lymphocytes (PubMed:16809611). Tumor-suppressor protein involved in T-cell lymphomas. May function on the P53-signaling pathway. Repress transcription through direct, TFCOUP2-independent binding to a GC-rich response element. {ECO:0000250|UniProtKB:Q9C0K0, ECO:0000269|PubMed:12717433, ECO:0000269|PubMed:16809611}.
- Poids moléculaire
- 94.6 kDa
- UniProt
- Q99PV8
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