MAGE-Like 2 Protein (MAGEL2) (AA 1-1284) (Strep Tag)
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- Antigène Voir toutes MAGE-Like 2 (MAGEL2) Protéines
- MAGE-Like 2 (MAGEL2)
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1284
- Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette MAGE-Like 2 protéine est marqué à la Strep Tag.
- Application
- ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Séquence
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MSQLSTNLGD SSPPESPVPA VHSRPTVLMR APPASSRAPP VPWDPPPVDL QAPMAAWQAP QPAWEAPEGQ LPAPVAQLAQ PPGLGAPMVQ APPLGGGMAK PPTPGVLMVH QPPPGAPMAQ SSTPGVLMLH PSVTGAPLAH PPPPGTPMTH PPGTSMAHPP PPPPPPPPPP PPGTPMTHPP PPGTPMGHHP PPGNPMTHPP PGNPMVHPLT HGAPMVHGGP HGTPMPHVPI TGTPIAQQPT PGVLMAQQLT PGVLMVQPPA PGAPMVQPPP QAALMTQPAP SITPMAKPPG PGVVMIHPPG ARGPIIQTPV SGAPMAQTVL PPGQPLATWA PQGQPLILQI QSQVIRAPPQ VPSVPQAPQV QLATPPGWQA TTPNWQVTPQ GWPATPLTWQ ATQVTWQAPT IAWQATQPGR QGHSTIRTGH TPIRPGPAPL LRQIPPMIRQ IQPVMRQAPP LIRQVPIRPA PHGIASQPQL WQVLPPPPPL RQAPQARLLL PRVPGTGQVS TVPPVAQIHL VPQSGPQVPQ TVLPAQLSIP IPVPQAAAQS APRTVHCPPI IWQAPKGQAP VPQELPVPQE LPVPQELPVP QEVPVPQEIP VPQEIPVPQE LPVPQELPVP QELPVPQELP VPQELPVPQE LPVPQELPVP QELPVPLEFQ EVQQAQAVGW RAPKVPPHFW QPVSAQEAQE QATQIAHVEQ QQPFQGAPAS SKALQTQLPT HQAQASGLQA ELPSVQLQPS WQGPLPMLQA QPGASATLAN FPRGSTRSRM APSGEPGPSS LEPRGPPRER RAPARDKKGP PKERMFIGAT FCAPRGASAS RAYVPTAWKN LPATSETFPA TSRVFPSTSH FQPASSNAFR GPSAASESPK SLPFALQDPY ACVEALPAVP WVPYPDGNAS SACKSVPAIL MVAAAAPQAS ATAAEASKSS EPPRRPGKAT RKKKHLEPKE DNCGHRLSSR DWRGPRTWGN PSHSDWEIQR AMQLLGDRES LYTPQGLNDW GCPNTSRMPR SLEGPSTSRD QEFCGDSGGS QTWMASEVPS VSRGSSAAQE DPDRESQPLS PLDERANALV QFLLVKDQAK VPVQLSEMVN VVIREYKDDS LDIINRANTK LECTFGCQLK EVDTKTHTYI IVNKMAYPQC NLLASYLERP KFSLLMVVLS LIFMKGYCIR ENLLFSFLFQ LGLDVQETSG LFRITKKLIT SVFVRHRYLE YRQIPFTEPA EYELLWGPRA FLETNRVHIL RFLAALYENQ PQIWSCQYLD SLAELEYKDA NAAAEESHDS DDDAHDPTSS PHPH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- MAGE-Like 2 (MAGEL2)
- Autre désignation
- Magel2 (MAGEL2 Produits)
- Synonymes
- NDNL1 Protein, nM15 Protein, MAGEL2 Protein, Mage-l2 Protein, ns7 Protein, MAGE family member L2 Protein, melanoma antigen, family L, 2 Protein, MAGEL2 Protein, Magel2 Protein
- Sujet
- MAGE-like protein 2 (Protein nS7),FUNCTION: Probably enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases, possibly through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Acts as a regulator of retrograde transport via its interaction with VPS35. Recruited to retromer-containing endosomes and promotes the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1 together with TRIM27, leading to promote endosomal F-actin assembly (By similarity). Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer. Significantly promotes the cytoplasmic accumulation of CLOCK (PubMed:22208286). {ECO:0000250|UniProtKB:Q9UJ55, ECO:0000269|PubMed:22208286}.
- Poids moléculaire
- 138.0 kDa
- UniProt
- Q9QZ04
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