Exonuclease 1 Protein (EXO1) (AA 1-837) (Strep Tag)
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- Antigène Voir toutes Exonuclease 1 (EXO1) Protéines
- Exonuclease 1 (EXO1)
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-837
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette Exonuclease 1 protéine est marqué à la Strep Tag.
- Application
- Western Blotting (WB), SDS-PAGE (SDS), ELISA
- Séquence
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MGIQGLLQFI QEASEPVNVK KYKGQAVAVD TYCWLHKGAI ACAEKLAKGE PTDRYVGFCM KFVNMLLSYG VKPILIFDGC TLPSKKEVER SRRERRQSNL LKGKQLLREG KVSEARDCFA RSINITHAMA HKVIKAARAL GVDCLVAPYE ADAQLAYLNK AGIVQAVITE DSDLLAFGCK KVILKMDQFG NGLEVDQARL GMCKQLGDVF TEEKFRYMCI LSGCDYLASL RGIGLAKACK VLRLANNPDI VKVIKKIGHY LRMNITVPED YITGFIRANN TFLYQLVFDP IQRKLVPLNA YGDDVNPETL TYAGQYVGDS VALQIALGNR DVNTFEQIDD YSPDTMPAHS RSHSWNEKAG QKPPGTNSIW HKNYCPRLEV NSVSHAPQLK EKPSTLGLKQ VISTKGLNLP RKSCVLKRPR NEALAEDDLL SQYSSVSKKI KENGCGDGTS PNSSKMSKSC PDSGTAHKTD AHTPSKMRNK FATFLQRRNE ESGAVVVPGT RSRFFCSSQD FDNFIPKKES GQPLNETVAT GKATTSLLGA LDCPDTEGHK PVDANGTHNL SSQIPGNAAV SPEDEAQSSE TSKLLGAMSP PSLGTLRSCF SWSGTLREFS RTPSPSASTT LQQFRRKSDP PACLPEASAV VTDRCDSKSE MLGETSQPLH ELGCSSRSQE SMDSSCGLNT SSLSQPSSRD SGSEESDCNN KSLDNQGEQN SKQHLPHFSK KDGLRRNKVP GLCRSSSMDS FSTTKIKPLV PARVSGLSKK SGSMQTRKHH DVENKPGLQT KISELWKNFG FKKDSEKLPS CKKPLSPVKD NIQLTPETED EIFNKPECVR AQRAIFH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
- Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
- Pureté
- > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
- Top Product
- Discover our top product EXO1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- Exonuclease 1 (EXO1)
- Autre désignation
- Exo1 (EXO1 Produits)
- Synonymes
- HEX1 Protein, hExoI Protein, 5730442G03Rik Protein, Msa Protein, EXO1 Protein, exoi Protein, fc16e04 Protein, wu:fc16e04 Protein, zgc:55521 Protein, exonuclease 1 Protein, hypothetical protein Protein, exonuclease I Exo1 Protein, RNA exonuclease Protein, exonuclease 1 S homeolog Protein, EXO1 Protein, Y17G7B.12 Protein, SJAG_01832 Protein, PAS_chr1-1_0377 Protein, Exo1 Protein, exo1 Protein, exo1.S Protein, LOC100338764 Protein
- Sujet
- Exonuclease 1 (mExo1) (EC 3.1.-.-) (Exonuclease I),FUNCTION: 5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis. {ECO:0000269|PubMed:12629043, ECO:0000269|PubMed:14716311}.
- Poids moléculaire
- 92.0 kDa
- UniProt
- Q9QZ11
- Pathways
- Réparation de l'ADN, Production of Molecular Mediator of Immune Response
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